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CHECK report for debrowser on malbec2

This page was generated on 2019-10-16 12:08:34 -0400 (Wed, 16 Oct 2019).

Package 396/1741HostnameOS / ArchINSTALLBUILDCHECKBUILD BIN
debrowser 1.12.3
Alper Kucukural
Snapshot Date: 2019-10-15 17:01:26 -0400 (Tue, 15 Oct 2019)
URL: https://git.bioconductor.org/packages/debrowser
Branch: RELEASE_3_9
Last Commit: a21fc72
Last Changed Date: 2019-08-09 16:02:14 -0400 (Fri, 09 Aug 2019)
malbec2 Linux (Ubuntu 18.04.2 LTS) / x86_64  OK  OK [ OK ]UNNEEDED, same version exists in internal repository
tokay2 Windows Server 2012 R2 Standard / x64  OK  OK  OK  OK UNNEEDED, same version exists in internal repository
celaya2 OS X 10.11.6 El Capitan / x86_64  OK  OK  OK  OK UNNEEDED, same version exists in internal repository

Summary

Package: debrowser
Version: 1.12.3
Command: /home/biocbuild/bbs-3.9-bioc/R/bin/R CMD check --install=check:debrowser.install-out.txt --library=/home/biocbuild/bbs-3.9-bioc/R/library --no-vignettes --timings debrowser_1.12.3.tar.gz
StartedAt: 2019-10-16 00:59:47 -0400 (Wed, 16 Oct 2019)
EndedAt: 2019-10-16 01:06:22 -0400 (Wed, 16 Oct 2019)
EllapsedTime: 395.3 seconds
RetCode: 0
Status:  OK 
CheckDir: debrowser.Rcheck
Warnings: 0

Command output

##############################################################################
##############################################################################
###
### Running command:
###
###   /home/biocbuild/bbs-3.9-bioc/R/bin/R CMD check --install=check:debrowser.install-out.txt --library=/home/biocbuild/bbs-3.9-bioc/R/library --no-vignettes --timings debrowser_1.12.3.tar.gz
###
##############################################################################
##############################################################################


* using log directory ‘/home/biocbuild/bbs-3.9-bioc/meat/debrowser.Rcheck’
* using R version 3.6.1 (2019-07-05)
* using platform: x86_64-pc-linux-gnu (64-bit)
* using session charset: UTF-8
* using option ‘--no-vignettes’
* checking for file ‘debrowser/DESCRIPTION’ ... OK
* checking extension type ... Package
* this is package ‘debrowser’ version ‘1.12.3’
* package encoding: UTF-8
* checking package namespace information ... OK
* checking package dependencies ... OK
* checking if this is a source package ... OK
* checking if there is a namespace ... OK
* checking for hidden files and directories ... OK
* checking for portable file names ... OK
* checking for sufficient/correct file permissions ... OK
* checking whether package ‘debrowser’ can be installed ... OK
* checking installed package size ... OK
* checking package directory ... OK
* checking ‘build’ directory ... OK
* checking DESCRIPTION meta-information ... OK
* checking top-level files ... OK
* checking for left-over files ... OK
* checking index information ... OK
* checking package subdirectories ... OK
* checking R files for non-ASCII characters ... OK
* checking R files for syntax errors ... OK
* checking whether the package can be loaded ... OK
* checking whether the package can be loaded with stated dependencies ... OK
* checking whether the package can be unloaded cleanly ... OK
* checking whether the namespace can be loaded with stated dependencies ... OK
* checking whether the namespace can be unloaded cleanly ... OK
* checking dependencies in R code ... OK
* checking S3 generic/method consistency ... OK
* checking replacement functions ... OK
* checking foreign function calls ... OK
* checking R code for possible problems ... NOTE
checkCountData : : no visible binding for global variable
  ‘err’
debrowserdataload: no visible binding for global variable ‘demodata’
debrowserhistogram: no visible global function definition for ‘config’
getBarMainPlot: no visible global function definition for ‘config’
getBoxMainPlot: no visible global function definition for ‘config’
getDensityPlot: no visible binding for global variable ‘samples’
getDensityPlot: no visible global function definition for ‘config’
getIQRPlot: no visible global function definition for ‘config’
getMean: no visible binding for global variable ‘norm_data’
mainScatterNew: no visible global function definition for ‘config’
plot_pca: no visible binding for global variable ‘x’
plot_pca: no visible binding for global variable ‘y’
plot_pca: no visible binding for global variable ‘samples’
plot_pca: no visible global function definition for ‘config’
runHeatmap: no visible global function definition for ‘config’
Undefined global functions or variables:
  config demodata err norm_data samples x y
* checking Rd files ... OK
* checking Rd metadata ... OK
* checking Rd cross-references ... OK
* checking for missing documentation entries ... OK
* checking for code/documentation mismatches ... OK
* checking Rd \usage sections ... OK
* checking Rd contents ... OK
* checking for unstated dependencies in examples ... OK
* checking files in ‘vignettes’ ... OK
* checking examples ... OK
* checking for unstated dependencies in ‘tests’ ... OK
* checking tests ...
  Running ‘test-demo.R’
  Running ‘test-deseq.R’
  Running ‘test-null.R’
  Running ‘test-ui.R’
 OK
* checking for unstated dependencies in vignettes ... OK
* checking package vignettes in ‘inst/doc’ ... OK
* checking running R code from vignettes ... SKIPPED
* checking re-building of vignette outputs ... SKIPPED
* checking PDF version of manual ... OK
* DONE

Status: 1 NOTE
See
  ‘/home/biocbuild/bbs-3.9-bioc/meat/debrowser.Rcheck/00check.log’
for details.



Installation output

debrowser.Rcheck/00install.out

##############################################################################
##############################################################################
###
### Running command:
###
###   /home/biocbuild/bbs-3.9-bioc/R/bin/R CMD INSTALL debrowser
###
##############################################################################
##############################################################################


* installing to library ‘/home/biocbuild/bbs-3.9-bioc/R/library’
* installing *source* package ‘debrowser’ ...
** using staged installation
** R
** inst
** byte-compile and prepare package for lazy loading
** help
*** installing help indices
** building package indices
** installing vignettes
** testing if installed package can be loaded from temporary location
** testing if installed package can be loaded from final location
** testing if installed package keeps a record of temporary installation path
* DONE (debrowser)

Tests output

debrowser.Rcheck/tests/test-demo.Rout


R version 3.6.1 (2019-07-05) -- "Action of the Toes"
Copyright (C) 2019 The R Foundation for Statistical Computing
Platform: x86_64-pc-linux-gnu (64-bit)

R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.

R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.

Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.

> require(shiny)
Loading required package: shiny
> library(debrowser)



##############################################################################
Pathview is an open source software package distributed under GNU General
Public License version 3 (GPLv3). Details of GPLv3 is available at
http://www.gnu.org/licenses/gpl-3.0.html. Particullary, users are required to
formally cite the original Pathview paper (not just mention it) in publications
or products. For details, do citation("pathview") within R.

The pathview downloads and uses KEGG data. Non-academic uses may require a KEGG
license agreement (details at http://www.kegg.jp/kegg/legal.html).
##############################################################################
DEBrowser v1.12.3  For help: https://debrowser.readthedocs.org/

If you use DEBrowser in published research, please cite:

Alper Kucukural, Onur Yukselen, Deniz M. Ozata, Melissa J. Moore, Manuel Garber
DEBrowser: Interactive Differential Expression Analysis and Visualization Tool for Count Data
BMC Genomics 2019 20:6

doi:0.1186/s12864-018-5362-x

> library(testthat)
> 
> test_that("demo data can be loaded", {
+     load(system.file("extdata", "demo", "demodata.Rda",
+         package = "debrowser"))
+     expect_true(is.data.frame(demodata))
+     expect_equal(demodata[29311, 2], 2)
+     expect_equal(demodata[29311, 5], 7.1)
+     expect_equal(demodata[29311, 6], 6)
+     expect_null(demodata[1, 7])
+ })
> 
> proc.time()
   user  system elapsed 
 13.529   0.500  14.013 

debrowser.Rcheck/tests/test-deseq.Rout


R version 3.6.1 (2019-07-05) -- "Action of the Toes"
Copyright (C) 2019 The R Foundation for Statistical Computing
Platform: x86_64-pc-linux-gnu (64-bit)

R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.

R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.

Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.

> library(debrowser)



##############################################################################
Pathview is an open source software package distributed under GNU General
Public License version 3 (GPLv3). Details of GPLv3 is available at
http://www.gnu.org/licenses/gpl-3.0.html. Particullary, users are required to
formally cite the original Pathview paper (not just mention it) in publications
or products. For details, do citation("pathview") within R.

The pathview downloads and uses KEGG data. Non-academic uses may require a KEGG
license agreement (details at http://www.kegg.jp/kegg/legal.html).
##############################################################################
DEBrowser v1.12.3  For help: https://debrowser.readthedocs.org/

If you use DEBrowser in published research, please cite:

Alper Kucukural, Onur Yukselen, Deniz M. Ozata, Melissa J. Moore, Manuel Garber
DEBrowser: Interactive Differential Expression Analysis and Visualization Tool for Count Data
BMC Genomics 2019 20:6

doi:0.1186/s12864-018-5362-x

> library(testthat)
> 
> load(system.file("extdata", "demo", "demodata.Rda",
+     package = "debrowser"))
> columns <- c("exper_rep1", "exper_rep2", "exper_rep3",
+     "control_rep1", "control_rep2", "control_rep3")
> conds <- factor( c("Control", "Control", "Control",
+     "Treat", "Treat", "Treat") )
> data <- data.frame(demodata[, columns])
> params <-
+         #Run DESeq2 with the following parameters
+         c("DESeq2", "parametric", F, "Wald") 
> non_expressed_cutoff <- 10
> data <- subset(data, rowSums(data) > 10)
> test_that("Able to run DESeq2", {
+     deseqrun <- runDE(data, columns, conds, params)
+     expect_true(exists("deseqrun"))
+ })
> 
> ##################################################
> deseqrun <- runDE(data, columns, conds, params)
estimating size factors
estimating dispersions
gene-wise dispersion estimates
mean-dispersion relationship
final dispersion estimates
fitting model and testing
> 
> de_res <- data.frame(deseqrun)
> norm_data <- getNormalizedMatrix(data[, columns])
> rdata <- cbind(rownames(de_res), norm_data[rownames(de_res), columns],
+                 log10(rowMeans(norm_data[rownames(de_res),
+                 paste(c("exper_rep1", "exper_rep2", "exper_rep3"))])
+                 + 0.1), log10( rowMeans( norm_data[ rownames( de_res ),
+                 paste(c("control_rep1", "control_rep2", "control_rep3"))])
+                 + 0.1), de_res[rownames(de_res),
+                 c("padj", "log2FoldChange")], 2 ^ de_res[rownames(de_res),
+                 "log2FoldChange"], -1 *
+                 log10(de_res[rownames(de_res), "padj"]))
> colnames(rdata) <- c("ID", columns, "Cond1", "Cond2", "padj",
+                 "log2FoldChange", "foldChange", "log10padj")
> rdata <- as.data.frame(rdata)
> rdata$padj[is.na(rdata$padj)] <- 1
> 
> padj_cutoff <- 0.01
> foldChange_cutoff <- 2
> 
> rdata$Legend <- character(nrow(rdata))
> rdata$Legend[rdata$log2FoldChange > log2(foldChange_cutoff) &
+         rdata$padj < padj_cutoff] <- "Up"
> rdata$Legend[rdata$log2FoldChange < log2(1 / foldChange_cutoff) &
+         rdata$padj < padj_cutoff] <- "Down"
> rdata$Legend[abs(rdata$log2FoldChange) <= 
+         log2(foldChange_cutoff)] <- "NS"
> rdata$Legend[is.null(rdata$log10padj)] <- "NA"
> rdata$Size <- character(nrow(rdata))
> rdata[, "Size"] <- "40"
> 
> dat <- rdata
> dat$M <- rdata$Cond1 - rdata$Cond2
> dat$A <- (rdata$Cond1 + rdata$Cond2) / 2
> 
> updown <- rdata[rdata$Legend=="Up" | rdata$Legend=="Down",columns]
> ##################################################
> 
> test_that("Check the QC plots", {
+     expect_silent( all2all(data) )
+ })
> 
> 
> proc.time()
   user  system elapsed 
 38.561   0.629  39.182 

debrowser.Rcheck/tests/test-null.Rout


R version 3.6.1 (2019-07-05) -- "Action of the Toes"
Copyright (C) 2019 The R Foundation for Statistical Computing
Platform: x86_64-pc-linux-gnu (64-bit)

R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.

R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.

Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.

> require(shiny)
Loading required package: shiny
> library(debrowser)



##############################################################################
Pathview is an open source software package distributed under GNU General
Public License version 3 (GPLv3). Details of GPLv3 is available at
http://www.gnu.org/licenses/gpl-3.0.html. Particullary, users are required to
formally cite the original Pathview paper (not just mention it) in publications
or products. For details, do citation("pathview") within R.

The pathview downloads and uses KEGG data. Non-academic uses may require a KEGG
license agreement (details at http://www.kegg.jp/kegg/legal.html).
##############################################################################
DEBrowser v1.12.3  For help: https://debrowser.readthedocs.org/

If you use DEBrowser in published research, please cite:

Alper Kucukural, Onur Yukselen, Deniz M. Ozata, Melissa J. Moore, Manuel Garber
DEBrowser: Interactive Differential Expression Analysis and Visualization Tool for Count Data
BMC Genomics 2019 20:6

doi:0.1186/s12864-018-5362-x

> library(testthat)
> 
> test_that("passing no data returns NULL", {
+     expect_null(compareClust() )
+     expect_null(getGOPlots(NULL, NULL))
+     null_deseq <- runDE(NULL)
+     expect_null(null_deseq)
+     expect_null(plot_pca(NULL))
+ })
> 
> proc.time()
   user  system elapsed 
 15.995   0.596  16.576 

debrowser.Rcheck/tests/test-ui.Rout


R version 3.6.1 (2019-07-05) -- "Action of the Toes"
Copyright (C) 2019 The R Foundation for Statistical Computing
Platform: x86_64-pc-linux-gnu (64-bit)

R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.

R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.

Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.

> require(shiny)
Loading required package: shiny
> library(debrowser)



##############################################################################
Pathview is an open source software package distributed under GNU General
Public License version 3 (GPLv3). Details of GPLv3 is available at
http://www.gnu.org/licenses/gpl-3.0.html. Particullary, users are required to
formally cite the original Pathview paper (not just mention it) in publications
or products. For details, do citation("pathview") within R.

The pathview downloads and uses KEGG data. Non-academic uses may require a KEGG
license agreement (details at http://www.kegg.jp/kegg/legal.html).
##############################################################################
DEBrowser v1.12.3  For help: https://debrowser.readthedocs.org/

If you use DEBrowser in published research, please cite:

Alper Kucukural, Onur Yukselen, Deniz M. Ozata, Melissa J. Moore, Manuel Garber
DEBrowser: Interactive Differential Expression Analysis and Visualization Tool for Count Data
BMC Genomics 2019 20:6

doi:0.1186/s12864-018-5362-x

> library(testthat)
> 
> test_that("able to create panel UI", {
+     expect_silent( QCPanel <- getQCPanel() )
+     expect_true(exists("QCPanel"))
+     expect_equal(QCPanel[[1]][[1]], "div")
+ 
+     expect_silent( downloads <- getDownloadSection() )
+     expect_true(exists("downloads"))
+     expect_equal(downloads[[1]][[1]], "div")
+ 
+     expect_silent( getMain <- getMainPanel() )
+     expect_true(exists("getMain"))
+     expect_equal(getMain[[1]][[1]], "div")
+ 
+     expect_silent( getStart <- getStartupMsg() )
+     expect_true(exists("getStart"))
+     expect_equal(getStart[[1]][[1]], "div")
+ 
+     expect_silent( getAfter <- getAfterLoadMsg() )
+     expect_true(exists("getAfter"))
+     expect_equal(getAfter[[1]][[1]], "div")
+ 
+     expect_silent(getGO <- getGoPanel() )
+     expect_true(exists("getGO"))
+     expect_equal(getGO[[1]][[1]], "div")
+ })
> 
> proc.time()
   user  system elapsed 
 11.610   0.416  12.012 

Example timings

debrowser.Rcheck/debrowser-Ex.timings

nameusersystemelapsed
BoxMainPlotControlsUI0.0130.0000.014
IQRPlotControlsUI0.0010.0000.002
actionButtonDE0.0050.0000.005
addDataCols0.0000.0000.001
addID0.0000.0000.001
all2all0.1890.0000.190
all2allControlsUI0.0020.0000.003
applyFilters0.0000.0000.001
applyFiltersNew0.0000.0000.001
applyFiltersToMergedComparison000
barMainPlotControlsUI0.0010.0000.001
batchEffectUI0.0520.0040.056
batchMethod0.0000.0020.002
changeClusterOrder0.0000.0000.001
checkCountData0.0000.0010.000
checkMetaData000
clustFunParamsUI000
clusterData0.0000.0000.001
compareClust000
condSelectUI0.0040.0000.004
correctCombat000
correctHarman000
customColorsUI0.0070.0020.022
cutOffSelectionUI0.0050.0000.005
dataLCFUI0.0290.0040.034
dataLoadUI0.0220.0040.026
deServer0.0090.0040.014
deUI0.2020.0480.367
debrowserIQRplot0.0010.0000.000
debrowserall2all000
debrowserbarmainplot000
debrowserbatcheffect0.0000.0000.001
debrowserboxmainplot0.0000.0000.001
debrowsercondselect0.0010.0000.000
debrowserdataload0.0010.0000.001
debrowserdeanalysis0.0010.0000.000
debrowserdensityplot000
debrowserheatmap000
debrowserhistogram000
debrowserlowcountfilter000
debrowsermainplot0.1050.0000.105
debrowserpcaplot0.0010.0000.001
dendControlsUI0.0060.0000.007
densityPlotControlsUI0.0010.0000.001
distFunParamsUI000
drawKEGG0.0010.0000.000
drawPCAExplained000
fileTypes000
fileUploadBox0.0030.0000.003
generateTestData000
getAfterLoadMsg0.0010.0000.000
getAll2AllPlotUI000
getBSTableUI000
getBarMainPlot0.0000.0000.001
getBarMainPlotUI0.0000.0000.001
getBoxMainPlot0.0010.0000.000
getBoxMainPlotUI0.0010.0000.000
getColorShapeSelection000
getColors0.0000.0000.001
getCompSelection0.0000.0020.002
getCondMsg0.0010.0000.000
getConditionSelector000
getConditionSelectorFromMeta0.0020.0000.002
getCutOffSelection0.0010.0000.001
getDEAnalysisText0.0010.0000.001
getDEResultsUI0.0010.0000.001
getDataAssesmentText0.0010.0000.001
getDataForTables000
getDataPreparationText0.0010.0000.001
getDensityPlot0.0010.0000.000
getDensityPlotUI000
getDomains000
getDown000
getDownloadSection0.0080.0000.007
getEnrichDO000
getEnrichGO000
getEnrichKEGG0.0000.0000.001
getEntrezIds0.0010.0000.000
getEntrezTable000
getGOLeftMenu0.0090.0000.009
getGOPlots0.0010.0000.000
getGeneList1.1010.0281.128
getGeneSetData0.0010.0000.000
getGoPanel0.0210.0000.020
getHeatmapUI000
getHelpButton000
getHideLegendOnOff0.0020.0000.002
getHistogramUI000
getIQRPlot000
getIQRPlotUI000
getIntroText0.0010.0000.001
getJSLine0.0130.0000.013
getKEGGModal0.0020.0000.003
getLeftMenu0.0000.0000.001
getLegendColors000
getLegendRadio0.0020.0000.002
getLegendSelect0.0020.0000.002
getLevelOrder0.0010.0000.000
getLoadingMsg0.0020.0000.001
getLogo0.0010.0000.001
getMainPanel0.0010.0000.001
getMainPlotUI000
getMainPlotsLeftMenu0.0820.0000.082
getMean0.0000.0000.001
getMergedComparison0.0000.0010.001
getMetaSelector0.0000.0000.001
getMethodDetails0.0010.0000.001
getMostVariedList0.0010.0000.000
getNormalizedMatrix0.0230.0000.023
getOrganism0.0010.0000.000
getOrganismBox0.0040.0000.004
getOrganismPathway0.0000.0000.001
getPCAPlotUI0.0010.0000.000
getPCAcontolUpdatesJS0.0000.0000.001
getPCAexplained1.6080.0561.676
getPCselection0.0010.0000.001
getPlotArea0.0010.0000.001
getProgramTitle000
getQAText0.0010.0000.001
getQCLeftMenu0.0010.0000.001
getQCPanel0.0030.0000.003
getSampleDetails000
getSampleNames000
getSamples000
getSearchData000
getSelHeat000
getSelectInputBox000
getSelectedCols000
getSelectedDatasetInput000
getShapeColor000
getStartPlotsMsg0.0020.0000.002
getStartupMsg0.0010.0000.001
getTabUpdateJS000
getTableDetails000
getTableModal0.0080.0000.008
getTableStyle000
getTextOnOff0.0020.0000.002
getUp000
getUpDown000
getVariationData000
get_conditions_given_selection0.0010.0000.001
heatmapControlsUI0.030.000.03
heatmapJScode0.0010.0000.001
heatmapServer0.0010.0000.001
heatmapUI0.070.000.07
hideObj0.0010.0000.001
histogramControlsUI0.0010.0000.000
installpack000
kmeansControlsUI0.0040.0000.005
lcfMetRadio0.0010.0000.002
loadpack0.0100.0080.018
mainPlotControlsUI0.0040.0000.004
mainScatterNew000
niceKmeans000
normalizationMethods0.0020.0000.002
palUI0.0020.0000.002
panel.cor0.0000.0010.000
panel.hist0.0000.0010.001
pcaPlotControlsUI0.0060.0020.007
plotData0.0000.0000.001
plotMarginsUI0.0030.0000.003
plotSizeMarginsUI0.0050.0000.005
plotSizeUI0.0020.0000.002
plotTypeUI000
plot_pca0.9000.0080.909
prepDEOutput000
prepDataContainer0.0010.0000.001
prepGroup0.0010.0000.000
prepHeatData000
prepPCADat000
push0.0010.0000.000
removeCols000
removeExtraCols0.0010.0000.001
round_vals0.0010.0000.000
runDE000
runDESeq2000
runEdgeR0.0000.0000.001
runHeatmap0.0000.0000.001
runHeatmap20.0000.0000.001
runLimma0.0010.0000.001
run_pca1.2650.0001.265
selectConditions0.0000.0000.001
selectGroupInfo0.0000.0000.001
selectedInput000
sepRadio0.0020.0000.001
setBatch000
showObj000
startDEBrowser0.0010.0000.000
startHeatmap000
textareaInput000
togglePanels0.0000.0000.001