Generating an using Ensembl based annotation packages
20 November 2019
Package
ensembldb 2.10.2
1 Introduction
The ensembldb package provides functions to create and use transcript centric
annotation databases/packages. The annotation for the databases are directly
fetched from Ensembl 1 using their Perl API. The functionality and data is
similar to that of the TxDb packages from the GenomicFeatures package, but, in
addition to retrieve all gene/transcript models and annotations from the
database, the ensembldb package provides also a filter framework allowing to
retrieve annotations for specific entries like genes encoded on a chromosome
region or transcript models of lincRNA genes. From version 1.7 on, EnsDb
databases created by the ensembldb package contain also protein annotation data
(see Section 11 for the database layout and an overview of
available attributes/columns). For more information on the use of the protein
annotations refer to the proteins vignette.
Another main goal of this package is to generate versioned annotation
packages, i.e. annotation packages that are build for a specific Ensembl
release, and are also named according to that (e.g. EnsDb.Hsapiens.v86 for
human gene definitions of the Ensembl code database version 86). This ensures
reproducibility, as it allows to load annotations from a specific Ensembl
release also if newer versions of annotation packages/releases are available. It
also allows to load multiple annotation packages at the same time in order to
e.g. compare gene models between Ensembl releases.
In the example below we load an Ensembl based annotation package for Homo
sapiens, Ensembl version 86. The EnsDb object providing access to the underlying
SQLite database is bound to the variable name EnsDb.Hsapiens.v86.
library(EnsDb.Hsapiens.v86)
## Making a "short cut"
edb <- EnsDb.Hsapiens.v86
## print some informations for this package
edb## EnsDb for Ensembl:
## |Backend: SQLite
## |Db type: EnsDb
## |Type of Gene ID: Ensembl Gene ID
## |Supporting package: ensembldb
## |Db created by: ensembldb package from Bioconductor
## |script_version: 0.3.0
## |Creation time: Thu May 18 16:32:27 2017
## |ensembl_version: 86
## |ensembl_host: localhost
## |Organism: homo_sapiens
## |taxonomy_id: 9606
## |genome_build: GRCh38
## |DBSCHEMAVERSION: 2.0
## | No. of genes: 63970.
## | No. of transcripts: 216741.
## |Protein data available.## For what organism was the database generated?
organism(edb)## [1] "Homo sapiens"2 Using ensembldb annotation packages to retrieve specific annotations
One of the strengths of the ensembldb package and the related EnsDb databases is
its implementation of a filter framework that enables to efficiently extract
data sub-sets from the databases. The ensembldb package supports most of the
filters defined in the AnnotationFilter Bioconductor package and defines some
additional filters specific to the data stored in EnsDb databases. Filters can
be passed directly to all methods extracting data from an EnsDb (such as genes,
transcripts or exons). Alternatively it is possible with the addFilter or filter
functions to add a filter directly to an EnsDb which will then be used in all
queries on that object.
The supportedFilters method can be used to get an overview over all supported
filter classes, each of them (except the GRangesFilter) working on a single
column/field in the database.
supportedFilters(edb)## filter field
## 1 EntrezFilter entrez
## 2 ExonEndFilter exon_end
## 3 ExonIdFilter exon_id
## 4 ExonRankFilter exon_rank
## 5 ExonStartFilter exon_start
## 6 GRangesFilter <NA>
## 7 GeneBiotypeFilter gene_biotype
## 8 GeneEndFilter gene_end
## 9 GeneIdFilter gene_id
## 10 GeneNameFilter gene_name
## 11 GeneStartFilter gene_start
## 12 GenenameFilter genename
## 13 ProtDomIdFilter prot_dom_id
## 14 ProteinDomainIdFilter protein_domain_id
## 15 ProteinDomainSourceFilter protein_domain_source
## 16 ProteinIdFilter protein_id
## 17 SeqNameFilter seq_name
## 18 SeqStrandFilter seq_strand
## 19 SymbolFilter symbol
## 20 TxBiotypeFilter tx_biotype
## 21 TxEndFilter tx_end
## 22 TxIdFilter tx_id
## 23 TxNameFilter tx_name
## 24 TxStartFilter tx_start
## 25 UniprotDbFilter uniprot_db
## 26 UniprotFilter uniprot
## 27 UniprotMappingTypeFilter uniprot_mapping_typeThese filters can be divided into 3 main filter types:
IntegerFilter: filter classes extending this basic object can take a single numeric value as input and support the conditions ==, !=, >, <, >= and <=. All filters that work on chromosomal coordinates, such as theGeneEndFilterextendIntegerFilter.CharacterFilter: filter classes extending this object can take a single or multiple character values as input and allow conditions: ==, !=, “startsWith” , “endsWith” and “contains”. All filters working on IDs extend this class.GRangesFilter: takes aGRangesobject as input and supports all conditions thatfindOverlapsfrom theIRangespackage supports (“any”, “start”, “end”, “within”, “equal”). Note that these have to be passed using the parametertypeto the constructor function.
The supported filters are:
EntrezFilter: allows to filter results based on NCBI Entrezgene identifiers of the genes.ExonEndFilter: filter using the chromosomal end coordinate of exons.ExonIdFilter: filter based on the (Ensembl) exon identifiers.ExonRankFilter: filter based on the rank (index) of an exon within the transcript model. Exons are always numbered from 5’ to 3’ end of the transcript, thus, also on the reverse strand, the exon 1 is the most 5’ exon of the transcript.ExonStartFilter: filter using the chromosomal start coordinate of exons.GeneBiotypeFilter: filter using the gene biotypes defined in the Ensembl database; use thelistGenebiotypesmethod to list all available biotypes.GeneEndFilter: filter using the chromosomal end coordinate of gene.GeneIdFilter: filter based on the Ensembl gene IDs.GeneNameFilter: filter based on the names (symbols) of the genes.GeneStartFilter: filter using the chromosomal start coordinate of gene.GRangesFilter: allows to retrieve all features (genes, transcripts or exons) that are either within (setting parametertypeto “within”) or partially overlapping (settingtypeto “any”) the defined genomic region/range. Note that, depending on the called method (genes,transcriptsorexons) the start and end coordinates of either the genes, transcripts or exons are used for the filter. For methodsexonsBy,cdsByandtxBythe coordinates ofbyare used.SeqNameFilter: filter by the name of the chromosomes the genes are encoded on.SeqStrandFilter: filter for the chromosome strand on which the genes are encoded.SymbolFilter: filter on gene symbols; note that no database columns symbol is available in anEnsDbdatabase and hence the gene name is used for filtering.TxBiotypeFilter: filter on the transcript biotype defined in Ensembl; use thelistTxbiotypesmethod to list all available biotypes.TxEndFilter: filter using the chromosomal end coordinate of transcripts.TxIdFilter: filter on the Ensembl transcript identifiers.TxNameFilter: filter on the Ensembl transcript names (currently identical to the transcript IDs).TxStartFilter: filter using the chromosomal start coordinate of transcripts.
In addition to the above listed DNA-RNA-based filters, protein-specific filters are also available:
ProtDomIdFilter,ProteinDomainIdFilter: filter by the protein domain ID.ProteinDomainSourceFilter: filter by the source of the protein domain (database or method, e.g. pfam).ProteinIdFilter: filter by Ensembl protein ID filters.UniprotDbFilter: filter by the name of the Uniprot database.UniprotFilter: filter by the Uniprot ID.UniprotMappingTypeFilter: filter by the mapping type of Ensembl protein IDs to Uniprot IDs.
These can however only be used on EnsDb databases that provide protein
annotations, i.e. for which a call to hasProteinData returns TRUE.
EnsDb databases for more recent Ensembl versions (starting from Ensembl 87)
provide also evidence levels for individual transcripts in the tx_support_level
database column. Such databases support also a TxSupportLevelFilter filter to
use this columns for filtering.
A simple use case for the filter framework would be to get all transcripts for
the gene BCL2L11. To this end we specify a GeneNameFilter with the value
BCL2L11. As a result we get a GRanges object with start, end, strand and seqname
being the start coordinate, end coordinate, chromosome name and strand for the
respective transcripts. All additional annotations are available as metadata
columns. Alternatively, by setting return.type to “DataFrame”, or “data.frame”
the method would return a DataFrame or data.frame object instead of the default
GRanges.
Tx <- transcripts(edb, filter = GeneNameFilter("BCL2L11"))
Tx## GRanges object with 28 ranges and 7 metadata columns:
## seqnames ranges strand | tx_id
## <Rle> <IRanges> <Rle> | <character>
## ENST00000432179 2 111119378-111124112 + | ENST00000432179
## ENST00000308659 2 111120914-111165048 + | ENST00000308659
## ENST00000337565 2 111120914-111128844 + | ENST00000337565
## ENST00000622509 2 111120914-111168445 + | ENST00000622509
## ENST00000619294 2 111120914-111168445 + | ENST00000619294
## ... ... ... ... . ...
## ENST00000452231 2 111123746-111164231 + | ENST00000452231
## ENST00000361493 2 111123746-111164231 + | ENST00000361493
## ENST00000431217 2 111123746-111164352 + | ENST00000431217
## ENST00000439718 2 111123746-111164643 + | ENST00000439718
## ENST00000438054 2 111123752-111146284 + | ENST00000438054
## tx_biotype tx_cds_seq_start tx_cds_seq_end
## <character> <integer> <integer>
## ENST00000432179 protein_coding 111123746 111124112
## ENST00000308659 protein_coding 111123746 111164231
## ENST00000337565 protein_coding 111123746 111128751
## ENST00000622509 protein_coding 111123746 111161439
## ENST00000619294 protein_coding 111123746 111144501
## ... ... ... ...
## ENST00000452231 nonsense_mediated_decay 111123746 111161439
## ENST00000361493 nonsense_mediated_decay 111123746 111130235
## ENST00000431217 nonsense_mediated_decay 111123746 111144501
## ENST00000439718 nonsense_mediated_decay 111123746 111151851
## ENST00000438054 protein_coding 111123752 111144491
## gene_id tx_name gene_name
## <character> <character> <character>
## ENST00000432179 ENSG00000153094 ENST00000432179 BCL2L11
## ENST00000308659 ENSG00000153094 ENST00000308659 BCL2L11
## ENST00000337565 ENSG00000153094 ENST00000337565 BCL2L11
## ENST00000622509 ENSG00000153094 ENST00000622509 BCL2L11
## ENST00000619294 ENSG00000153094 ENST00000619294 BCL2L11
## ... ... ... ...
## ENST00000452231 ENSG00000153094 ENST00000452231 BCL2L11
## ENST00000361493 ENSG00000153094 ENST00000361493 BCL2L11
## ENST00000431217 ENSG00000153094 ENST00000431217 BCL2L11
## ENST00000439718 ENSG00000153094 ENST00000439718 BCL2L11
## ENST00000438054 ENSG00000153094 ENST00000438054 BCL2L11
## -------
## seqinfo: 1 sequence from GRCh38 genome## as this is a GRanges object we can access e.g. the start coordinates with
head(start(Tx))## [1] 111119378 111120914 111120914 111120914 111120914 111120914## or extract the biotype with
head(Tx$tx_biotype)## [1] "protein_coding" "protein_coding" "protein_coding" "protein_coding"
## [5] "protein_coding" "protein_coding"The parameter columns of the extractor methods (such as exons, genes or
transcripts) allows to specify which database attributes (columns) should be
retrieved. The exons method returns by default all exon-related columns, the
transcripts all columns from the transcript database table and the genes all
from the gene table. Note however that in the example above we got also a column
gene_name although this column is not present in the transcript database
table. By default the methods return also all columns that are used by any of
the filters submitted with the filter argument (thus, because a GeneNameFilter
was used, the column gene_name is also returned). Setting
returnFilterColumns(edb) <- FALSE disables this option and only the columns
specified by the columns parameter are retrieved.
Instead of passing a filter object to the method it is also possible to provide
a filter expression written as a formula. The formula has to be written in the
form ~ <field> <condition> <value> with <field> being the field (database
column) in the database, <condition> the condition for the filter object and
<value> its value. Use the supportedFilter method to get the field names
corresponding to each filter class.
## Use a filter expression to perform the filtering.
transcripts(edb, filter = ~ gene_name == "ZBTB16")## GRanges object with 9 ranges and 7 metadata columns:
## seqnames ranges strand | tx_id
## <Rle> <IRanges> <Rle> | <character>
## ENST00000335953 11 114059593-114250676 + | ENST00000335953
## ENST00000541602 11 114059725-114189764 + | ENST00000541602
## ENST00000544220 11 114059737-114063646 + | ENST00000544220
## ENST00000535700 11 114060257-114063744 + | ENST00000535700
## ENST00000392996 11 114060507-114250652 + | ENST00000392996
## ENST00000539918 11 114064412-114247344 + | ENST00000539918
## ENST00000545851 11 114180766-114247296 + | ENST00000545851
## ENST00000535379 11 114237207-114250557 + | ENST00000535379
## ENST00000535509 11 114246790-114250476 + | ENST00000535509
## tx_biotype tx_cds_seq_start tx_cds_seq_end
## <character> <integer> <integer>
## ENST00000335953 protein_coding 114063301 114250555
## ENST00000541602 retained_intron <NA> <NA>
## ENST00000544220 protein_coding 114063301 114063646
## ENST00000535700 protein_coding 114063301 114063744
## ENST00000392996 protein_coding 114063301 114250555
## ENST00000539918 nonsense_mediated_decay 114064412 114121827
## ENST00000545851 processed_transcript <NA> <NA>
## ENST00000535379 processed_transcript <NA> <NA>
## ENST00000535509 retained_intron <NA> <NA>
## gene_id tx_name gene_name
## <character> <character> <character>
## ENST00000335953 ENSG00000109906 ENST00000335953 ZBTB16
## ENST00000541602 ENSG00000109906 ENST00000541602 ZBTB16
## ENST00000544220 ENSG00000109906 ENST00000544220 ZBTB16
## ENST00000535700 ENSG00000109906 ENST00000535700 ZBTB16
## ENST00000392996 ENSG00000109906 ENST00000392996 ZBTB16
## ENST00000539918 ENSG00000109906 ENST00000539918 ZBTB16
## ENST00000545851 ENSG00000109906 ENST00000545851 ZBTB16
## ENST00000535379 ENSG00000109906 ENST00000535379 ZBTB16
## ENST00000535509 ENSG00000109906 ENST00000535509 ZBTB16
## -------
## seqinfo: 1 sequence from GRCh38 genomeFilter expression have to be written as a formula (i.e. starting with a ~) in
the form column name followed by the logical condition.
Alternatively, EnsDb objects can be filtered directly using the filter
function. In the example below we use the filter function to filter the EnsDb
object and pass that filtered database to the transcripts method using the %>%
from the magrittr package.
library(magrittr)
edb %>% filter(~ symbol == "BCL2" & tx_biotype != "protein_coding") %>%
transcripts## GRanges object with 1 range and 6 metadata columns:
## seqnames ranges strand | tx_id
## <Rle> <IRanges> <Rle> | <character>
## ENST00000590515 18 63128212-63161869 - | ENST00000590515
## tx_biotype tx_cds_seq_start tx_cds_seq_end
## <character> <integer> <integer>
## ENST00000590515 processed_transcript <NA> <NA>
## gene_id tx_name
## <character> <character>
## ENST00000590515 ENSG00000171791 ENST00000590515
## -------
## seqinfo: 1 sequence from GRCh38 genomeAdding a filter to an EnsDb enables this filter (globally) on all subsequent
queries on that object. We could thus filter an EnsDb to (virtually) contain
only features encoded on chromosome Y.
edb_y <- addFilter(edb, SeqNameFilter("Y"))
## All subsequent filters on that EnsDb will only work on features encoded on
## chromosome Y
genes(edb_y)## GRanges object with 523 ranges and 6 metadata columns:
## seqnames ranges strand | gene_id
## <Rle> <IRanges> <Rle> | <character>
## ENSG00000251841 Y 2784749-2784853 + | ENSG00000251841
## ENSG00000184895 Y 2786855-2787699 - | ENSG00000184895
## ENSG00000237659 Y 2789827-2790328 + | ENSG00000237659
## ENSG00000232195 Y 2827982-2828218 + | ENSG00000232195
## ENSG00000129824 Y 2841486-2932000 + | ENSG00000129824
## ... ... ... ... . ...
## ENSG00000224240 Y 26549425-26549743 + | ENSG00000224240
## ENSG00000227629 Y 26586642-26591601 - | ENSG00000227629
## ENSG00000237917 Y 26594851-26634652 - | ENSG00000237917
## ENSG00000231514 Y 26626520-26627159 - | ENSG00000231514
## ENSG00000235857 Y 56855244-56855488 + | ENSG00000235857
## gene_name gene_biotype seq_coord_system
## <character> <character> <character>
## ENSG00000251841 RNU6-1334P snRNA chromosome
## ENSG00000184895 SRY protein_coding chromosome
## ENSG00000237659 RNASEH2CP1 processed_pseudogene chromosome
## ENSG00000232195 TOMM22P2 processed_pseudogene chromosome
## ENSG00000129824 RPS4Y1 protein_coding chromosome
## ... ... ... ...
## ENSG00000224240 CYCSP49 processed_pseudogene chromosome
## ENSG00000227629 SLC25A15P1 unprocessed_pseudogene chromosome
## ENSG00000237917 PARP4P1 unprocessed_pseudogene chromosome
## ENSG00000231514 FAM58CP processed_pseudogene chromosome
## ENSG00000235857 CTBP2P1 processed_pseudogene chromosome
## symbol entrezid
## <character> <list>
## ENSG00000251841 RNU6-1334P NA
## ENSG00000184895 SRY 6736
## ENSG00000237659 RNASEH2CP1 NA
## ENSG00000232195 TOMM22P2 NA
## ENSG00000129824 RPS4Y1 6192
## ... ... ...
## ENSG00000224240 CYCSP49 NA
## ENSG00000227629 SLC25A15P1 NA
## ENSG00000237917 PARP4P1 NA
## ENSG00000231514 FAM58CP NA
## ENSG00000235857 CTBP2P1 NA
## -------
## seqinfo: 1 sequence from GRCh38 genome## Get all lincRNAs on chromosome Y
genes(edb_y, filter = ~ gene_biotype == "lincRNA")## GRanges object with 52 ranges and 6 metadata columns:
## seqnames ranges strand | gene_id
## <Rle> <IRanges> <Rle> | <character>
## ENSG00000278847 Y 2934406-2934771 - | ENSG00000278847
## ENSG00000231535 Y 3002912-3102272 + | ENSG00000231535
## ENSG00000229308 Y 4036497-4100320 + | ENSG00000229308
## ENSG00000277930 Y 4993858-4999650 - | ENSG00000277930
## ENSG00000237069 Y 6242446-6243610 - | ENSG00000237069
## ... ... ... ... . ...
## ENSG00000228296 Y 25063083-25099892 - | ENSG00000228296
## ENSG00000223641 Y 25183643-25184773 - | ENSG00000223641
## ENSG00000228786 Y 25378300-25394719 - | ENSG00000228786
## ENSG00000240450 Y 25482908-25486705 + | ENSG00000240450
## ENSG00000231141 Y 25728490-25733388 + | ENSG00000231141
## gene_name gene_biotype seq_coord_system symbol
## <character> <character> <character> <character>
## ENSG00000278847 RP11-414C23.1 lincRNA chromosome RP11-414C23.1
## ENSG00000231535 LINC00278 lincRNA chromosome LINC00278
## ENSG00000229308 AC010084.1 lincRNA chromosome AC010084.1
## ENSG00000277930 RP11-122L9.1 lincRNA chromosome RP11-122L9.1
## ENSG00000237069 TTTY23B lincRNA chromosome TTTY23B
## ... ... ... ... ...
## ENSG00000228296 TTTY4C lincRNA chromosome TTTY4C
## ENSG00000223641 TTTY17C lincRNA chromosome TTTY17C
## ENSG00000228786 LINC00266-4P lincRNA chromosome LINC00266-4P
## ENSG00000240450 CSPG4P1Y lincRNA chromosome CSPG4P1Y
## ENSG00000231141 TTTY3 lincRNA chromosome TTTY3
## entrezid
## <list>
## ENSG00000278847 NA
## ENSG00000231535 100873962
## ENSG00000229308 NA
## ENSG00000277930 NA
## ENSG00000237069 c(100101121, 252955)
## ... ...
## ENSG00000228296 c(474150, 474149, 114761)
## ENSG00000223641 c(474152, 474151, 252949)
## ENSG00000228786 NA
## ENSG00000240450 114758
## ENSG00000231141 c(474148, 114760)
## -------
## seqinfo: 1 sequence from GRCh38 genomeTo get an overview of database tables and available columns the function
listTables can be used. The method listColumns on the other hand lists columns
for the specified database table.
## list all database tables along with their columns
listTables(edb)## $gene
## [1] "gene_id" "gene_name" "gene_biotype" "gene_seq_start"
## [5] "gene_seq_end" "seq_name" "seq_strand" "seq_coord_system"
## [9] "symbol"
##
## $tx
## [1] "tx_id" "tx_biotype" "tx_seq_start" "tx_seq_end"
## [5] "tx_cds_seq_start" "tx_cds_seq_end" "gene_id" "tx_name"
##
## $tx2exon
## [1] "tx_id" "exon_id" "exon_idx"
##
## $exon
## [1] "exon_id" "exon_seq_start" "exon_seq_end"
##
## $chromosome
## [1] "seq_name" "seq_length" "is_circular"
##
## $protein
## [1] "tx_id" "protein_id" "protein_sequence"
##
## $uniprot
## [1] "protein_id" "uniprot_id" "uniprot_db"
## [4] "uniprot_mapping_type"
##
## $protein_domain
## [1] "protein_id" "protein_domain_id" "protein_domain_source"
## [4] "interpro_accession" "prot_dom_start" "prot_dom_end"
##
## $entrezgene
## [1] "gene_id" "entrezid"
##
## $metadata
## [1] "name" "value"## list columns from a specific table
listColumns(edb, "tx")## [1] "tx_id" "tx_biotype" "tx_seq_start" "tx_seq_end"
## [5] "tx_cds_seq_start" "tx_cds_seq_end" "gene_id" "tx_name"Thus, we could retrieve all transcripts of the biotype nonsense_mediated_decay
(which, according to the definitions by Ensembl are transcribed, but most likely
not translated in a protein, but rather degraded after transcription) along with
the name of the gene for each transcript. Note that we are changing here the
return.type to DataFrame, so the method will return a DataFrame with the
results instead of the default GRanges.
Tx <- transcripts(edb,
columns = c(listColumns(edb , "tx"), "gene_name"),
filter = TxBiotypeFilter("nonsense_mediated_decay"),
return.type = "DataFrame")
nrow(Tx)## [1] 14423Tx## DataFrame with 14423 rows and 9 columns
## tx_id tx_biotype tx_seq_start tx_seq_end
## <character> <character> <integer> <integer>
## 1 ENST00000567466 nonsense_mediated_decay 47578 49521
## 2 ENST00000397876 nonsense_mediated_decay 53887 57372
## 3 ENST00000428730 nonsense_mediated_decay 58062 65039
## 4 ENST00000417043 nonsense_mediated_decay 62973 65037
## 5 ENST00000622194 nonsense_mediated_decay 85386 138349
## ... ... ... ... ...
## 14419 ENST00000496411 nonsense_mediated_decay 248855728 248859018
## 14420 ENST00000483223 nonsense_mediated_decay 248856515 248858529
## 14421 ENST00000533647 nonsense_mediated_decay 248857273 248858324
## 14422 ENST00000528141 nonsense_mediated_decay 248857391 248859085
## 14423 ENST00000530986 nonsense_mediated_decay 248857469 248859085
## tx_cds_seq_start tx_cds_seq_end gene_id tx_name
## <integer> <integer> <character> <character>
## 1 48546 48893 ENSG00000261456 ENST00000567466
## 2 54017 56360 ENSG00000161981 ENST00000397876
## 3 62884 65015 ENSG00000007384 ENST00000428730
## 4 63904 65015 ENSG00000007384 ENST00000417043
## 5 117330 138267 ENSG00000103148 ENST00000622194
## ... ... ... ... ...
## 14419 248857954 248858309 ENSG00000171163 ENST00000496411
## 14420 248857954 248858309 ENSG00000171163 ENST00000483223
## 14421 248857954 248858309 ENSG00000171163 ENST00000533647
## 14422 248858004 248858309 ENSG00000171163 ENST00000528141
## 14423 248858004 248858309 ENSG00000171163 ENST00000530986
## gene_name
## <character>
## 1 TUBB8
## 2 SNRNP25
## 3 RHBDF1
## 4 RHBDF1
## 5 NPRL3
## ... ...
## 14419 ZNF692
## 14420 ZNF692
## 14421 ZNF692
## 14422 ZNF692
## 14423 ZNF692For protein coding transcripts, we can also specifically extract their coding region. In the example below we extract the CDS for all transcripts encoded on chromosome Y.
yCds <- cdsBy(edb, filter = SeqNameFilter("Y"))
yCds## GRangesList object of length 151:
## $ENST00000155093
## GRanges object with 7 ranges and 3 metadata columns:
## seqnames ranges strand | seq_name exon_id exon_rank
## <Rle> <IRanges> <Rle> | <character> <character> <integer>
## [1] Y 2953937-2953997 + | Y ENSE00002223884 2
## [2] Y 2961074-2961646 + | Y ENSE00003645989 3
## [3] Y 2975095-2975244 + | Y ENSE00003764421 4
## [4] Y 2975511-2975654 + | Y ENSE00003768468 5
## [5] Y 2976670-2976822 + | Y ENSE00003766362 6
## [6] Y 2977940-2978080 + | Y ENSE00003766086 7
## [7] Y 2978810-2979993 + | Y ENSE00001368923 8
## -------
## seqinfo: 1 sequence from GRCh38 genome
##
## $ENST00000215473
## GRanges object with 2 ranges and 3 metadata columns:
## seqnames ranges strand | seq_name exon_id exon_rank
## <Rle> <IRanges> <Rle> | <character> <character> <integer>
## [1] Y 5056824-5057459 + | Y ENSE00001436852 1
## [2] Y 5098215-5100740 + | Y ENSE00003741448 2
## -------
## seqinfo: 1 sequence from GRCh38 genome
##
## $ENST00000215479
## GRanges object with 5 ranges and 3 metadata columns:
## seqnames ranges strand | seq_name exon_id exon_rank
## <Rle> <IRanges> <Rle> | <character> <character> <integer>
## [1] Y 6872555-6872608 - | Y ENSE00001671586 2
## [2] Y 6870006-6870053 - | Y ENSE00001645681 3
## [3] Y 6868732-6868776 - | Y ENSE00000652250 4
## [4] Y 6868037-6868462 - | Y ENSE00001667251 5
## [5] Y 6866073-6866078 - | Y ENSE00001494454 6
## -------
## seqinfo: 1 sequence from GRCh38 genome
##
## ...
## <148 more elements>Using a GRangesFilter we can retrieve all features from the database that are
either within or overlapping the specified genomic region. In the example
below we query all genes that are partially overlapping with a small region on
chromosome 11. The filter restricts to all genes for which either an exon or an
intron is partially overlapping with the region.
## Define the filter
grf <- GRangesFilter(GRanges("11", ranges = IRanges(114129278, 114129328),
strand = "+"), type = "any")
## Query genes:
gn <- genes(edb, filter = grf)
gn## GRanges object with 1 range and 6 metadata columns:
## seqnames ranges strand | gene_id
## <Rle> <IRanges> <Rle> | <character>
## ENSG00000109906 11 114059593-114250676 + | ENSG00000109906
## gene_name gene_biotype seq_coord_system symbol
## <character> <character> <character> <character>
## ENSG00000109906 ZBTB16 protein_coding chromosome ZBTB16
## entrezid
## <list>
## ENSG00000109906 7704
## -------
## seqinfo: 1 sequence from GRCh38 genome## Next we retrieve all transcripts for that gene so that we can plot them.
txs <- transcripts(edb, filter = GeneNameFilter(gn$gene_name))
As we can see, 4 transcripts of the gene ZBTB16 are also overlapping the
region. Below we fetch these 4 transcripts. Note, that a call to exons will
not return any features from the database, as no exon is overlapping with the
region.
transcripts(edb, filter = grf)## GRanges object with 4 ranges and 6 metadata columns:
## seqnames ranges strand | tx_id
## <Rle> <IRanges> <Rle> | <character>
## ENST00000335953 11 114059593-114250676 + | ENST00000335953
## ENST00000541602 11 114059725-114189764 + | ENST00000541602
## ENST00000392996 11 114060507-114250652 + | ENST00000392996
## ENST00000539918 11 114064412-114247344 + | ENST00000539918
## tx_biotype tx_cds_seq_start tx_cds_seq_end
## <character> <integer> <integer>
## ENST00000335953 protein_coding 114063301 114250555
## ENST00000541602 retained_intron <NA> <NA>
## ENST00000392996 protein_coding 114063301 114250555
## ENST00000539918 nonsense_mediated_decay 114064412 114121827
## gene_id tx_name
## <character> <character>
## ENST00000335953 ENSG00000109906 ENST00000335953
## ENST00000541602 ENSG00000109906 ENST00000541602
## ENST00000392996 ENSG00000109906 ENST00000392996
## ENST00000539918 ENSG00000109906 ENST00000539918
## -------
## seqinfo: 1 sequence from GRCh38 genomeThe GRangesFilter supports also GRanges defining multiple regions and a
query will return all features overlapping any of these regions. Besides using
the GRangesFilter it is also possible to search for transcripts or exons
overlapping genomic regions using the exonsByOverlaps or
transcriptsByOverlaps known from the GenomicFeatures package. Note that the
implementation of these methods for EnsDb objects supports also to use filters
to further fine-tune the query.
The functions listGenebiotypes and listTxbiotypes can be used to get an overview
of allowed/available gene and transcript biotype
## Get all gene biotypes from the database. The GeneBiotypeFilter
## allows to filter on these values.
listGenebiotypes(edb)## [1] "protein_coding" "unitary_pseudogene"
## [3] "unprocessed_pseudogene" "processed_pseudogene"
## [5] "processed_transcript" "transcribed_unprocessed_pseudogene"
## [7] "antisense" "transcribed_unitary_pseudogene"
## [9] "polymorphic_pseudogene" "lincRNA"
## [11] "sense_intronic" "transcribed_processed_pseudogene"
## [13] "sense_overlapping" "IG_V_pseudogene"
## [15] "pseudogene" "TR_V_gene"
## [17] "3prime_overlapping_ncRNA" "IG_V_gene"
## [19] "bidirectional_promoter_lncRNA" "snRNA"
## [21] "miRNA" "misc_RNA"
## [23] "snoRNA" "rRNA"
## [25] "Mt_tRNA" "Mt_rRNA"
## [27] "IG_C_gene" "IG_J_gene"
## [29] "TR_J_gene" "TR_C_gene"
## [31] "TR_V_pseudogene" "TR_J_pseudogene"
## [33] "IG_D_gene" "ribozyme"
## [35] "IG_C_pseudogene" "TR_D_gene"
## [37] "TEC" "IG_J_pseudogene"
## [39] "scRNA" "scaRNA"
## [41] "vaultRNA" "sRNA"
## [43] "macro_lncRNA" "non_coding"
## [45] "IG_pseudogene" "LRG_gene"## Get all transcript biotypes from the database.
listTxbiotypes(edb)## [1] "protein_coding" "processed_transcript"
## [3] "nonsense_mediated_decay" "retained_intron"
## [5] "unitary_pseudogene" "TEC"
## [7] "miRNA" "misc_RNA"
## [9] "non_stop_decay" "unprocessed_pseudogene"
## [11] "processed_pseudogene" "transcribed_unprocessed_pseudogene"
## [13] "lincRNA" "antisense"
## [15] "transcribed_unitary_pseudogene" "polymorphic_pseudogene"
## [17] "sense_intronic" "transcribed_processed_pseudogene"
## [19] "sense_overlapping" "IG_V_pseudogene"
## [21] "pseudogene" "TR_V_gene"
## [23] "3prime_overlapping_ncRNA" "IG_V_gene"
## [25] "bidirectional_promoter_lncRNA" "snRNA"
## [27] "snoRNA" "rRNA"
## [29] "Mt_tRNA" "Mt_rRNA"
## [31] "IG_C_gene" "IG_J_gene"
## [33] "TR_J_gene" "TR_C_gene"
## [35] "TR_V_pseudogene" "TR_J_pseudogene"
## [37] "IG_D_gene" "ribozyme"
## [39] "IG_C_pseudogene" "TR_D_gene"
## [41] "IG_J_pseudogene" "scRNA"
## [43] "scaRNA" "vaultRNA"
## [45] "sRNA" "macro_lncRNA"
## [47] "non_coding" "IG_pseudogene"
## [49] "LRG_gene"Data can be fetched in an analogous way using the exons and genes
methods. In the example below we retrieve gene_name, entrezid and the
gene_biotype of all genes in the database which names start with “BCL2”.
## We're going to fetch all genes which names start with BCL.
BCLs <- genes(edb,
columns = c("gene_name", "entrezid", "gene_biotype"),
filter = GeneNameFilter("BCL", condition = "startsWith"),
return.type = "DataFrame")
nrow(BCLs)## [1] 30BCLs## DataFrame with 30 rows and 4 columns
## gene_name entrezid gene_biotype gene_id
## <character> <list> <character> <character>
## 1 BCL10 8915 protein_coding ENSG00000142867
## 2 BCL11A 53335 protein_coding ENSG00000119866
## 3 BCL11B 64919 protein_coding ENSG00000127152
## 4 BCL2 596 protein_coding ENSG00000171791
## 5 BCL2A1 597 protein_coding ENSG00000140379
## ... ... ... ... ...
## 26 BCL9L 283149 protein_coding ENSG00000186174
## 27 BCL9P1 NA processed_pseudogene ENSG00000249238
## 28 BCLAF1 9774 protein_coding ENSG00000029363
## 29 BCLAF1P1 NA processed_pseudogene ENSG00000248966
## 30 BCLAF1P2 NA processed_pseudogene ENSG00000279800Sometimes it might be useful to know the length of genes or transcripts
(i.e. the total sum of nucleotides covered by their exons). Below we calculate
the mean length of transcripts from protein coding genes on chromosomes X and Y
as well as the average length of snoRNA, snRNA and rRNA transcripts encoded on
these chromosomes. For the first query we combine two AnnotationFilter objects
using an AnnotationFilterList object, in the second we define the query using a
filter expression.
## determine the average length of snRNA, snoRNA and rRNA genes encoded on
## chromosomes X and Y.
mean(lengthOf(edb, of = "tx",
filter = AnnotationFilterList(
GeneBiotypeFilter(c("snRNA", "snoRNA", "rRNA")),
SeqNameFilter(c("X", "Y")))))## [1] 118.2458## determine the average length of protein coding genes encoded on the same
## chromosomes.
mean(lengthOf(edb, of = "tx",
filter = ~ gene_biotype == "protein_coding" &
seq_name %in% c("X", "Y")))## [1] 1943.554Not unexpectedly, transcripts of protein coding genes are longer than those of snRNA, snoRNA or rRNA genes.
At last we extract the first two exons of each transcript model from the database.
## Extract all exons 1 and (if present) 2 for all genes encoded on the
## Y chromosome
exons(edb, columns = c("tx_id", "exon_idx"),
filter = list(SeqNameFilter("Y"),
ExonRankFilter(3, condition = "<")))## GRanges object with 1294 ranges and 3 metadata columns:
## seqnames ranges strand | tx_id exon_idx
## <Rle> <IRanges> <Rle> | <character> <integer>
## ENSE00002088309 Y 2784749-2784853 + | ENST00000516032 1
## ENSE00001494622 Y 2786855-2787699 - | ENST00000383070 1
## ENSE00001772499 Y 2789827-2790328 + | ENST00000454281 1
## ENSE00001614266 Y 2827982-2828218 + | ENST00000430735 1
## ENSE00002490412 Y 2841486-2841627 + | ENST00000250784 1
## ... ... ... ... . ... ...
## ENSE00001632993 Y 26591548-26591601 - | ENST00000456738 1
## ENSE00001616687 Y 26626520-26627159 - | ENST00000435741 1
## ENSE00001638296 Y 26633345-26633431 - | ENST00000435945 2
## ENSE00001797328 Y 26634523-26634652 - | ENST00000435945 1
## ENSE00001794473 Y 56855244-56855488 + | ENST00000431853 1
## exon_id
## <character>
## ENSE00002088309 ENSE00002088309
## ENSE00001494622 ENSE00001494622
## ENSE00001772499 ENSE00001772499
## ENSE00001614266 ENSE00001614266
## ENSE00002490412 ENSE00002490412
## ... ...
## ENSE00001632993 ENSE00001632993
## ENSE00001616687 ENSE00001616687
## ENSE00001638296 ENSE00001638296
## ENSE00001797328 ENSE00001797328
## ENSE00001794473 ENSE00001794473
## -------
## seqinfo: 1 sequence from GRCh38 genome3 Extracting gene/transcript/exon models for RNASeq feature counting
For the feature counting step of an RNAseq experiment, the gene or transcript
models (defined by the chromosomal start and end positions of their exons) have
to be known. To extract these from an Ensembl based annotation package, the
exonsBy, genesBy and transcriptsBy methods can be used in an analogous way as in
TxDb packages generated by the GenomicFeatures package. However, the
transcriptsBy method does not, in contrast to the method in the GenomicFeatures
package, allow to return transcripts by “cds”. While the annotation packages
built by the ensembldb contain the chromosomal start and end coordinates of
the coding region (for protein coding genes) they do not assign an ID to each
CDS.
A simple use case is to retrieve all genes encoded on chromosomes X and Y from the database.
TxByGns <- transcriptsBy(edb, by = "gene", filter = SeqNameFilter(c("X", "Y")))
TxByGns## GRangesList object of length 2922:
## $ENSG00000000003
## GRanges object with 5 ranges and 6 metadata columns:
## seqnames ranges strand | tx_id
## <Rle> <IRanges> <Rle> | <character>
## [1] X 100633442-100639991 - | ENST00000494424
## [2] X 100627109-100637104 - | ENST00000612152
## [3] X 100632063-100637104 - | ENST00000614008
## [4] X 100628670-100636806 - | ENST00000373020
## [5] X 100632541-100636689 - | ENST00000496771
## tx_biotype tx_cds_seq_start tx_cds_seq_end gene_id
## <character> <integer> <integer> <character>
## [1] processed_transcript <NA> <NA> ENSG00000000003
## [2] protein_coding 100630798 100635569 ENSG00000000003
## [3] protein_coding 100632063 100635569 ENSG00000000003
## [4] protein_coding 100630798 100636694 ENSG00000000003
## [5] processed_transcript <NA> <NA> ENSG00000000003
## tx_name
## <character>
## [1] ENST00000494424
## [2] ENST00000612152
## [3] ENST00000614008
## [4] ENST00000373020
## [5] ENST00000496771
## -------
## seqinfo: 2 sequences from GRCh38 genome
##
## $ENSG00000000005
## GRanges object with 2 ranges and 6 metadata columns:
## seqnames ranges strand | tx_id
## <Rle> <IRanges> <Rle> | <character>
## [1] X 100584802-100599885 + | ENST00000373031
## [2] X 100593624-100597531 + | ENST00000485971
## tx_biotype tx_cds_seq_start tx_cds_seq_end gene_id
## <character> <integer> <integer> <character>
## [1] protein_coding 100585019 100599717 ENSG00000000005
## [2] processed_transcript <NA> <NA> ENSG00000000005
## tx_name
## <character>
## [1] ENST00000373031
## [2] ENST00000485971
## -------
## seqinfo: 2 sequences from GRCh38 genome
##
## $ENSG00000001497
## GRanges object with 5 ranges and 6 metadata columns:
## seqnames ranges strand | tx_id
## <Rle> <IRanges> <Rle> | <character>
## [1] X 65512583-65534775 - | ENST00000484069
## [2] X 65512582-65534756 - | ENST00000374811
## [3] X 65512583-65534756 - | ENST00000374804
## [4] X 65512582-65534754 - | ENST00000374807
## [5] X 65520429-65523617 - | ENST00000469091
## tx_biotype tx_cds_seq_start tx_cds_seq_end gene_id
## <character> <integer> <integer> <character>
## [1] nonsense_mediated_decay 65525021 65534715 ENSG00000001497
## [2] protein_coding 65512775 65534715 ENSG00000001497
## [3] protein_coding 65512775 65534715 ENSG00000001497
## [4] protein_coding 65512775 65534715 ENSG00000001497
## [5] protein_coding 65520655 65523617 ENSG00000001497
## tx_name
## <character>
## [1] ENST00000484069
## [2] ENST00000374811
## [3] ENST00000374804
## [4] ENST00000374807
## [5] ENST00000469091
## -------
## seqinfo: 2 sequences from GRCh38 genome
##
## ...
## <2919 more elements>Since Ensembl contains also definitions of genes that are on chromosome variants (supercontigs), it is advisable to specify the chromosome names for which the gene models should be returned.
In a real use case, we might thus want to retrieve all genes encoded on the
standard chromosomes. In addition it is advisable to use a GeneIdFilter to
restrict to Ensembl genes only, as also LRG (Locus Reference Genomic)
genes2 are defined in the database, which are partially redundant with
Ensembl genes.
## will just get exons for all genes on chromosomes 1 to 22, X and Y.
## Note: want to get rid of the "LRG" genes!!!
EnsGenes <- exonsBy(edb, by = "gene", filter = AnnotationFilterList(
SeqNameFilter(c(1:22, "X", "Y")),
GeneIdFilter("ENSG", "startsWith")))The code above returns a GRangesList that can be used directly as an input for
the summarizeOverlaps function from the GenomicAlignments package 3.
Alternatively, the above GRangesList can be transformed to a data.frame in
SAF format that can be used as an input to the featureCounts function of the
Rsubread package 4.
## Transforming the GRangesList into a data.frame in SAF format
EnsGenes.SAF <- toSAF(EnsGenes)Note that the ID by which the GRangesList is split is used in the SAF
formatted data.frame as the GeneID. In the example below this would be the
Ensembl gene IDs, while the start, end coordinates (along with the strand and
chromosomes) are those of the the exons.
In addition, the disjointExons function (similar to the one defined in
GenomicFeatures) can be used to generate a GRanges of non-overlapping exon
parts which can be used in the DEXSeq package.
## Create a GRanges of non-overlapping exon parts.
DJE <- disjointExons(edb, filter = AnnotationFilterList(
SeqNameFilter(c(1:22, "X", "Y")),
GeneIdFilter("ENSG%", "startsWith")))4 Retrieving sequences for gene/transcript/exon models
The methods to retrieve exons, transcripts and genes (i.e. exons, transcripts
and genes) return by default GRanges objects that can be used to retrieve
sequences using the getSeq method e.g. from BSgenome packages. The basic
workflow is thus identical to the one for TxDb packages, however, it is not
straight forward to identify the BSgenome package with the matching genomic
sequence. Most BSgenome packages are named according to the genome build
identifier used in UCSC which does not (always) match the genome build name used
by Ensembl. Using the Ensembl version provided by the EnsDb, the correct genomic
sequence can however be retrieved easily from the AnnotationHub using the
getGenomeTeoBitFile. If no 2bit file matching the Ensembl version is available,
the function tries to identify a file with the correct genome build from the
closest Ensembl release and returns that instead.
In the code block below we retrieve first the TwoBitFile with the genomic DNA
sequence, extract the genomic start and end coordinates for all genes defined in
the package, subset to genes encoded on sequences available in the TwoBitFile
and extract all of their sequences. Note: these sequences represent the sequence
between the chromosomal start and end coordinates of the gene.
library(EnsDb.Hsapiens.v86)
edb <- EnsDb.Hsapiens.v86
## Get the TwoBit with the genomic sequence matching the Ensembl version
## using the AnnotationHub package.
dna <- ensembldb:::getGenomeTwoBitFile(edb)
## Get start/end coordinates of all genes.
genes <- genes(edb)
## Subset to all genes that are encoded on chromosomes for which
## we do have DNA sequence available.
genes <- genes[seqnames(genes) %in% seqnames(seqinfo(dna))]
## Get the gene sequences, i.e. the sequence including the sequence of
## all of the gene's exons and introns.
geneSeqs <- getSeq(dna, genes)To retrieve the (exonic) sequence of transcripts (i.e. without introns) we can
use directly the extractTranscriptSeqs method defined in the GenomicFeatures on
the EnsDb object, eventually using a filter to restrict the query.
## get all exons of all transcripts encoded on chromosome Y
yTx <- exonsBy(edb, filter = SeqNameFilter("Y"))
## Retrieve the sequences for these transcripts from the TwoBitile.
library(GenomicFeatures)
yTxSeqs <- extractTranscriptSeqs(dna, yTx)
yTxSeqs
## Extract the sequences of all transcripts encoded on chromosome Y.
yTx <- extractTranscriptSeqs(dna, edb, filter = SeqNameFilter("Y"))
## Along these lines, we could use the method also to retrieve the coding sequence
## of all transcripts on the Y chromosome.
cdsY <- cdsBy(edb, filter = SeqNameFilter("Y"))
extractTranscriptSeqs(dna, cdsY)Next we retrieve transcript sequences from genes encoded on chromosome Y using
the BSGenome package for the human genome. Ensembl version 86 based on
the GRCh38 genome build and we thus load the corresponding BSGenome package.
library(BSgenome.Hsapiens.NCBI.GRCh38)
bsg <- BSgenome.Hsapiens.NCBI.GRCh38
## Get the genome version
unique(genome(bsg))## [1] "GRCh38"unique(genome(edb))## [1] "GRCh38"## Extract the full transcript sequences.
yTxSeqs <- extractTranscriptSeqs(
bsg, exonsBy(edb, "tx", filter = SeqNameFilter("Y")))
yTxSeqs## A DNAStringSet instance of length 740
## width seq names
## [1] 5239 GCCTAGTGCGCGCGCAGTAACC...AATAAATGTTTACTTGTATATG ENST00000155093
## [2] 4595 CTGGTGGTCCAGTACCTCCAAA...TGAGCCCTTCAGAAGACATTCT ENST00000215473
## [3] 802 AGAGGACCAAGCCTCCCTGTGT...CAATAAAATGTTTTAAAAATCA ENST00000215479
## [4] 910 TGTCTGTCAGAGCTGTCAGCCT...TAAACACTGGTATATTTCTGTT ENST00000250776
## [5] 1305 TTCCAGGATATGAACTCTACAG...TAAATCCTGTGGCTGTAGGAAA ENST00000250784
## ... ... ...
## [736] 792 ATGGCCCGGGGCCCCAAGAAGC...TGCCAAACAGAGCAGTGGCTAA ENST00000629237
## [737] 344 GGTTGCCACTTCAAGGGACTAC...CTGGCTCTTCTGGCAGTTTTTT ENST00000631331
## [738] 933 CTCTCCCAGCTTCTACCCACAG...GCATACTATAAAAATGCTTTAA ENST00000634531
## [739] 1832 ATGTCTGCTGCAAATCCTGAGA...AGTATTTAAATCTGTTGGATCC ENST00000634662
## [740] 890 CTCTCCCAGCTTCTACCCACAG...GCATACTATAAAAATGCTTTAA ENST00000635343## Extract just the CDS
Test <- cdsBy(edb, "tx", filter = SeqNameFilter("Y"))
yTxCds <- extractTranscriptSeqs(
bsg, cdsBy(edb, "tx", filter = SeqNameFilter("Y")))
yTxCds## A DNAStringSet instance of length 151
## width seq names
## [1] 2406 ATGGATGAAGATGAATTTGAAT...TAAAGAAGTTGGTCTGCCCTAA ENST00000155093
## [2] 3162 ATGTTTAGGGTTGGCTTCTTAA...AGTTTCTAACACAACTTTCTAA ENST00000215473
## [3] 579 ATGGGGACCTGGATTTTGTTTG...CAAGCAGGAGGAAGTGGATTAA ENST00000215479
## [4] 792 ATGGCCCGGGGCCCCAAGAAGC...CACCAAACAGAGCAGTGGCTAA ENST00000250784
## [5] 378 ATGAGTCCAAAGCCGAGAGCCT...ATCTACTCCCCTATCTCCCTGA ENST00000250823
## ... ... ...
## [147] 387 ATGCAAAGCCAGAGAGGTCTCC...CACACTCTGTGTCCCAAAATGA ENST00000624507
## [148] 78 ATGAGAGCCAAGTGGAGGAAGA...GATGAGGCAGAAGTCCAAGTAA ENST00000624575
## [149] 1833 ATGGATGAAGATGAATTTGAAT...TAAAGAAGTTGGTCTGCCCTAA ENST00000625061
## [150] 792 ATGGCCCGGGGCCCCAAGAAGC...TGCCAAACAGAGCAGTGGCTAA ENST00000629237
## [151] 1740 ATGTCTGCTGCAAATCCTGAGA...TTTAATCCAGAGAAGAGACTGA ENST000006346625 Integrating annotations from Ensembl based EnsDb packages with UCSC based annotations
Sometimes it might be useful to combine (Ensembl based) annotations from EnsDb
packages/objects with annotations from other Bioconductor packages, that might
base on UCSC annotations. To support such an integration of annotations, the
ensembldb packages implements the seqlevelsStyle and seqlevelsStyle<- from the
GenomeInfoDb package that allow to change the style of chromosome naming. Thus,
sequence/chromosome names other than those used by Ensembl can be used in, and
are returned by, the queries to EnsDb objects as long as a mapping for them is
provided by the GenomeInfoDb package (which provides a mapping mostly between
UCSC, NCBI and Ensembl chromosome names for the main chromosomes).
In the example below we change the seqnames style to UCSC.
## Change the seqlevels style form Ensembl (default) to UCSC:
seqlevelsStyle(edb) <- "UCSC"
## Now we can use UCSC style seqnames in SeqNameFilters or GRangesFilter:
genesY <- genes(edb, filter = ~ seq_name == "chrY")
## The seqlevels of the returned GRanges are also in UCSC style
seqlevels(genesY)## [1] "chrY"Note that in most instances no mapping is available for sequences not
corresponding to the main chromosomes (i.e. contigs, patched chromosomes
etc). What is returned in cases in which no mapping is available can be
specified with the global ensembldb.seqnameNotFound option. By default (with
ensembldb.seqnameNotFound set to “ORIGINAL”), the original seqnames (i.e. the
ones from Ensembl) are returned. With ensembldb.seqnameNotFound “MISSING” each
time a seqname can not be found an error is thrown. For all other cases
(e.g. ensembldb.seqnameNotFound = NA) the value of the option is returned.
seqlevelsStyle(edb) <- "UCSC"
## Getting the default option:
getOption("ensembldb.seqnameNotFound")## [1] "ORIGINAL"## Listing all seqlevels in the database.
seqlevels(edb)[1:30]## Warning in .formatSeqnameByStyleFromQuery(x, sn, ifNotFound): More than 5
## seqnames with seqlevels style of the database (Ensembl) could not be mapped to
## the seqlevels style: UCSC!) Returning the orginal seqnames for these.## [1] "chr1" "chr10"
## [3] "chr11" "chr12"
## [5] "chr13" "chr14"
## [7] "chr15" "chr16"
## [9] "chr17" "chr18"
## [11] "chr19" "chr2"
## [13] "chr20" "chr21"
## [15] "chr22" "chr3"
## [17] "chr4" "chr5"
## [19] "chr6" "chr7"
## [21] "chr8" "chr9"
## [23] "CHR_HG107_PATCH" "CHR_HG126_PATCH"
## [25] "CHR_HG1311_PATCH" "CHR_HG1342_HG2282_PATCH"
## [27] "CHR_HG1362_PATCH" "CHR_HG142_HG150_NOVEL_TEST"
## [29] "CHR_HG151_NOVEL_TEST" "CHR_HG1651_PATCH"## Setting the option to NA, thus, for each seqname for which no mapping is available,
## NA is returned.
options(ensembldb.seqnameNotFound=NA)
seqlevels(edb)[1:30]## Warning in .formatSeqnameByStyleFromQuery(x, sn, ifNotFound): More than 5
## seqnames with seqlevels style of the database (Ensembl) could not be mapped to
## the seqlevels style: UCSC!) Returning NA for these.## [1] "chr1" "chr10" "chr11" "chr12" "chr13" "chr14" "chr15" "chr16" "chr17"
## [10] "chr18" "chr19" "chr2" "chr20" "chr21" "chr22" "chr3" "chr4" "chr5"
## [19] "chr6" "chr7" "chr8" "chr9" NA NA NA NA NA
## [28] NA NA NA## Resetting the option.
options(ensembldb.seqnameNotFound = "ORIGINAL")At last changing the seqname style to the default value "Ensembl".
seqlevelsStyle(edb) <- "Ensembl"6 Interactive annotation lookup using the shiny web app
In addition to the genes, transcripts and exons methods it is possibly to
search interactively for gene/transcript/exon annotations using the internal,
shiny based, web application. The application can be started with the
runEnsDbApp() function. The search results from this app can also be returned
to the R workspace either as a data.frame or GRanges object.
7 Plotting gene/transcript features using ensembldb and Gviz and ggbio
The Gviz package provides functions to plot genes and transcripts along with
other data on a genomic scale. Gene models can be provided either as a
data.frame, GRanges, TxDB database, can be fetched from biomart and can
also be retrieved from ensembldb.
Below we generate a GeneRegionTrack fetching all transcripts from a certain
region on chromosome Y.
Note that if we want in addition to work also with BAM files that were aligned
against DNA sequences retrieved from Ensembl or FASTA files representing genomic
DNA sequences from Ensembl we should change the ucscChromosomeNames option from
Gviz to FALSE (i.e. by calling options(ucscChromosomeNames = FALSE)). This is
not necessary if we just want to retrieve gene models from an EnsDb object, as
the ensembldb package internally checks the ucscChromosomeNames option and,
depending on that, maps Ensembl chromosome names to UCSC chromosome names.
## Loading the Gviz library
library(Gviz)
library(EnsDb.Hsapiens.v86)
edb <- EnsDb.Hsapiens.v86
## Retrieving a Gviz compatible GRanges object with all genes
## encoded on chromosome Y.
gr <- getGeneRegionTrackForGviz(edb, chromosome = "Y",
start = 20400000, end = 21400000)
## Define a genome axis track
gat <- GenomeAxisTrack()
## We have to change the ucscChromosomeNames option to FALSE to enable Gviz usage
## with non-UCSC chromosome names.
options(ucscChromosomeNames = FALSE)
plotTracks(list(gat, GeneRegionTrack(gr)))
options(ucscChromosomeNames = TRUE)Above we had to change the option ucscChromosomeNames to FALSE in order to
use it with non-UCSC chromosome names. Alternatively, we could however also
change the seqnamesStyle of the EnsDb object to UCSC. Note that we have to
use now also chromosome names in the UCSC style in the SeqNameFilter
(i.e. “chrY” instead of “Y”).
seqlevelsStyle(edb) <- "UCSC"
## Retrieving the GRanges objects with seqnames corresponding to UCSC chromosome names.
gr <- getGeneRegionTrackForGviz(edb, chromosome = "chrY",
start = 20400000, end = 21400000)## Warning in .formatSeqnameByStyleForQuery(x, sn, ifNotFound): Seqnames: Y could
## not be mapped to the seqlevels style of the database (Ensembl)!Returning the
## orginal seqnames for these.seqnames(gr)## factor-Rle of length 89 with 1 run
## Lengths: 89
## Values : chrY
## Levels(1): chrY## Define a genome axis track
gat <- GenomeAxisTrack()
plotTracks(list(gat, GeneRegionTrack(gr)))
We can also use the filters from the ensembldb package to further refine what
transcripts are fetched, like in the example below, in which we create two
different gene region tracks, one for protein coding genes and one for lincRNAs.
protCod <- getGeneRegionTrackForGviz(edb, chromosome = "chrY",
start = 20400000, end = 21400000,
filter = GeneBiotypeFilter("protein_coding"))
lincs <- getGeneRegionTrackForGviz(edb, chromosome = "chrY",
start = 20400000, end = 21400000,
filter = GeneBiotypeFilter("lincRNA"))
plotTracks(list(gat, GeneRegionTrack(protCod, name = "protein coding"),
GeneRegionTrack(lincs, name = "lincRNAs")), transcriptAnnotation = "symbol")
## At last we change the seqlevels style again to Ensembl
seqlevelsStyle <- "Ensembl"Alternatively, we can also use ggbio for plotting. For ggbio we can directly
pass the EnsDb object along with optional filters (or as in the example below a
filter expression as a formula).
library(ggbio)
## Create a plot for all transcripts of the gene SKA2
autoplot(edb, ~ gene_name == "SKA2")To plot the genomic region and plot genes from both strands we can use a
GRangesFilter.
## Get the chromosomal region in which the gene is encoded
ska2 <- genes(edb, filter = ~ gene_name == "SKA2")
strand(ska2) <- "*"
autoplot(edb, GRangesFilter(ska2), names.expr = "gene_name")8 Using EnsDb objects in the AnnotationDbi framework
Most of the methods defined for objects extending the basic annotation package
class AnnotationDbi are also defined for EnsDb objects (i.e. methods
columns, keytypes, keys, mapIds and select). While these methods can
be used analogously to basic annotation packages, the implementation for EnsDb
objects also support the filtering framework of the ensembldb package.
In the example below we first evaluate all the available columns and keytypes in the database and extract then the gene names for all genes encoded on chromosome X.
library(EnsDb.Hsapiens.v86)
edb <- EnsDb.Hsapiens.v86
## List all available columns in the database.
columns(edb)## [1] "ENTREZID" "EXONID" "EXONIDX"
## [4] "EXONSEQEND" "EXONSEQSTART" "GENEBIOTYPE"
## [7] "GENEID" "GENENAME" "GENESEQEND"
## [10] "GENESEQSTART" "INTERPROACCESSION" "ISCIRCULAR"
## [13] "PROTDOMEND" "PROTDOMSTART" "PROTEINDOMAINID"
## [16] "PROTEINDOMAINSOURCE" "PROTEINID" "PROTEINSEQUENCE"
## [19] "SEQCOORDSYSTEM" "SEQLENGTH" "SEQNAME"
## [22] "SEQSTRAND" "SYMBOL" "TXBIOTYPE"
## [25] "TXCDSSEQEND" "TXCDSSEQSTART" "TXID"
## [28] "TXNAME" "TXSEQEND" "TXSEQSTART"
## [31] "UNIPROTDB" "UNIPROTID" "UNIPROTMAPPINGTYPE"## Note that these do *not* correspond to the actual column names
## of the database that can be passed to methods like exons, genes,
## transcripts etc. These column names can be listed with the listColumns
## method.
listColumns(edb)## [1] "seq_name" "seq_length" "is_circular"
## [4] "gene_id" "entrezid" "exon_id"
## [7] "exon_seq_start" "exon_seq_end" "gene_name"
## [10] "gene_biotype" "gene_seq_start" "gene_seq_end"
## [13] "seq_strand" "seq_coord_system" "symbol"
## [16] "name" "value" "tx_id"
## [19] "protein_id" "protein_sequence" "protein_domain_id"
## [22] "protein_domain_source" "interpro_accession" "prot_dom_start"
## [25] "prot_dom_end" "tx_biotype" "tx_seq_start"
## [28] "tx_seq_end" "tx_cds_seq_start" "tx_cds_seq_end"
## [31] "tx_name" "exon_idx" "uniprot_id"
## [34] "uniprot_db" "uniprot_mapping_type"## List all of the supported key types.
keytypes(edb)## [1] "ENTREZID" "EXONID" "GENEBIOTYPE"
## [4] "GENEID" "GENENAME" "PROTDOMID"
## [7] "PROTEINDOMAINID" "PROTEINDOMAINSOURCE" "PROTEINID"
## [10] "SEQNAME" "SEQSTRAND" "SYMBOL"
## [13] "TXBIOTYPE" "TXID" "TXNAME"
## [16] "UNIPROTID"## Get all gene ids from the database.
gids <- keys(edb, keytype = "GENEID")
length(gids)## [1] 63970## Get all gene names for genes encoded on chromosome Y.
gnames <- keys(edb, keytype = "GENENAME", filter = SeqNameFilter("Y"))
head(gnames)## [1] "KDM5D" "DDX3Y" "ZFY" "TBL1Y" "PCDH11Y" "AMELY"In the next example we retrieve specific information from the database using the
select method. First we fetch all transcripts for the genes BCL2 and
BCL2L11. In the first call we provide the gene names, while in the second call
we employ the filtering system to perform a more fine-grained query to fetch
only the protein coding transcripts for these genes.
## Use the /standard/ way to fetch data.
select(edb, keys = c("BCL2", "BCL2L11"), keytype = "GENENAME",
columns = c("GENEID", "GENENAME", "TXID", "TXBIOTYPE"))## GENEID GENENAME TXID TXBIOTYPE
## 1 ENSG00000171791 BCL2 ENST00000398117 protein_coding
## 2 ENSG00000171791 BCL2 ENST00000333681 protein_coding
## 3 ENSG00000171791 BCL2 ENST00000590515 processed_transcript
## 4 ENSG00000171791 BCL2 ENST00000589955 protein_coding
## 5 ENSG00000153094 BCL2L11 ENST00000432179 protein_coding
## 6 ENSG00000153094 BCL2L11 ENST00000308659 protein_coding
## 7 ENSG00000153094 BCL2L11 ENST00000393256 protein_coding
## 8 ENSG00000153094 BCL2L11 ENST00000393252 protein_coding
## 9 ENSG00000153094 BCL2L11 ENST00000433098 nonsense_mediated_decay
## 10 ENSG00000153094 BCL2L11 ENST00000405953 protein_coding
## 11 ENSG00000153094 BCL2L11 ENST00000415458 nonsense_mediated_decay
## 12 ENSG00000153094 BCL2L11 ENST00000436733 nonsense_mediated_decay
## 13 ENSG00000153094 BCL2L11 ENST00000437029 nonsense_mediated_decay
## 14 ENSG00000153094 BCL2L11 ENST00000452231 nonsense_mediated_decay
## 15 ENSG00000153094 BCL2L11 ENST00000361493 nonsense_mediated_decay
## 16 ENSG00000153094 BCL2L11 ENST00000431217 nonsense_mediated_decay
## 17 ENSG00000153094 BCL2L11 ENST00000439718 nonsense_mediated_decay
## 18 ENSG00000153094 BCL2L11 ENST00000438054 protein_coding
## 19 ENSG00000153094 BCL2L11 ENST00000337565 protein_coding
## 20 ENSG00000153094 BCL2L11 ENST00000622509 protein_coding
## 21 ENSG00000153094 BCL2L11 ENST00000619294 protein_coding
## 22 ENSG00000153094 BCL2L11 ENST00000610735 protein_coding
## 23 ENSG00000153094 BCL2L11 ENST00000622612 protein_coding
## 24 ENSG00000153094 BCL2L11 ENST00000357757 protein_coding
## 25 ENSG00000153094 BCL2L11 ENST00000615946 protein_coding
## 26 ENSG00000153094 BCL2L11 ENST00000621302 protein_coding
## 27 ENSG00000153094 BCL2L11 ENST00000620862 protein_coding
## 28 LRG_620 BCL2L11 LRG_620t1 LRG_gene
## 29 LRG_620 BCL2L11 LRG_620t2 LRG_gene
## 30 LRG_620 BCL2L11 LRG_620t3 LRG_gene
## 31 LRG_620 BCL2L11 LRG_620t4 LRG_gene
## 32 LRG_620 BCL2L11 LRG_620t5 LRG_gene## Use the filtering system of ensembldb
select(edb, keys = ~ gene_name %in% c("BCL2", "BCL2L11") &
tx_biotype == "protein_coding",
columns = c("GENEID", "GENENAME", "TXID", "TXBIOTYPE"))## GENEID GENENAME TXID TXBIOTYPE
## 1 ENSG00000171791 BCL2 ENST00000398117 protein_coding
## 2 ENSG00000171791 BCL2 ENST00000333681 protein_coding
## 3 ENSG00000171791 BCL2 ENST00000589955 protein_coding
## 4 ENSG00000153094 BCL2L11 ENST00000432179 protein_coding
## 5 ENSG00000153094 BCL2L11 ENST00000308659 protein_coding
## 6 ENSG00000153094 BCL2L11 ENST00000393256 protein_coding
## 7 ENSG00000153094 BCL2L11 ENST00000393252 protein_coding
## 8 ENSG00000153094 BCL2L11 ENST00000405953 protein_coding
## 9 ENSG00000153094 BCL2L11 ENST00000438054 protein_coding
## 10 ENSG00000153094 BCL2L11 ENST00000337565 protein_coding
## 11 ENSG00000153094 BCL2L11 ENST00000622509 protein_coding
## 12 ENSG00000153094 BCL2L11 ENST00000619294 protein_coding
## 13 ENSG00000153094 BCL2L11 ENST00000610735 protein_coding
## 14 ENSG00000153094 BCL2L11 ENST00000622612 protein_coding
## 15 ENSG00000153094 BCL2L11 ENST00000357757 protein_coding
## 16 ENSG00000153094 BCL2L11 ENST00000615946 protein_coding
## 17 ENSG00000153094 BCL2L11 ENST00000621302 protein_coding
## 18 ENSG00000153094 BCL2L11 ENST00000620862 protein_codingFinally, we use the mapIds method to establish a mapping between ids and
values. In the example below we fetch transcript ids for the two genes from the
example above.
## Use the default method, which just returns the first value for multi mappings.
mapIds(edb, keys = c("BCL2", "BCL2L11"), column = "TXID", keytype = "GENENAME")## BCL2 BCL2L11
## "ENST00000398117" "ENST00000432179"## Alternatively, specify multiVals="list" to return all mappings.
mapIds(edb, keys = c("BCL2", "BCL2L11"), column = "TXID", keytype = "GENENAME",
multiVals = "list")## $BCL2
## [1] "ENST00000398117" "ENST00000333681" "ENST00000590515" "ENST00000589955"
##
## $BCL2L11
## [1] "ENST00000432179" "ENST00000308659" "ENST00000393256" "ENST00000393252"
## [5] "ENST00000433098" "ENST00000405953" "ENST00000415458" "ENST00000436733"
## [9] "ENST00000437029" "ENST00000452231" "ENST00000361493" "ENST00000431217"
## [13] "ENST00000439718" "ENST00000438054" "ENST00000337565" "ENST00000622509"
## [17] "ENST00000619294" "ENST00000610735" "ENST00000622612" "ENST00000357757"
## [21] "ENST00000615946" "ENST00000621302" "ENST00000620862" "LRG_620t1"
## [25] "LRG_620t2" "LRG_620t3" "LRG_620t4" "LRG_620t5"## And, just like before, we can use filters to map only to protein coding transcripts.
mapIds(edb, keys = list(GeneNameFilter(c("BCL2", "BCL2L11")),
TxBiotypeFilter("protein_coding")), column = "TXID",
multiVals = "list")## Warning in .mapIds(x = x, keys = keys, column = column, keytype = keytype, : Got
## 2 filter objects. Will use the keys of the first for the mapping!## $BCL2
## [1] "ENST00000398117" "ENST00000333681" "ENST00000589955"
##
## $BCL2L11
## [1] "ENST00000432179" "ENST00000308659" "ENST00000393256" "ENST00000393252"
## [5] "ENST00000405953" "ENST00000438054" "ENST00000337565" "ENST00000622509"
## [9] "ENST00000619294" "ENST00000610735" "ENST00000622612" "ENST00000357757"
## [13] "ENST00000615946" "ENST00000621302" "ENST00000620862"Note that, if the filters are used, the ordering of the result does no longer match the ordering of the genes.
9 Important notes
These notes might explain eventually unexpected results (and, more importantly, help avoiding them):
The ordering of the results returned by the
genes,exons,transcriptsmethods can be specified with theorder.byparameter. The ordering of the results does however not correspond to the ordering of values in submitted filter objects. The exception is theselectmethod. If a character vector of values or a single filter is passed with argumentkeysthe ordering of results of this method matches the ordering of the key values or the values of the filter.Results of
exonsBy,transcriptsByare always ordered by thebyargument.The CDS provided by
EnsDbobjects always includes both, the start and the stop codon.Transcripts with multiple CDS are at present not supported by
EnsDb.At present,
EnsDbsupport only genes/transcripts for which all of their exons are encoded on the same chromosome and the same strand.Since a single Ensembl gene ID might be mapped to multiple NCBI Entrezgene IDs methods such as
genes,transcriptsetc return alistin the"entrezid"column of the resulting result object.
10 Getting or building EnsDb databases/packages
Some of the code in this section is not supposed to be automatically executed
when the vignette is built, as this would require a working installation of the
Ensembl Perl API, which is not expected to be available on each system. Also,
building EnsDb from alternative sources, like GFF or GTF files takes some time
and thus also these examples are not directly executed when the vignette is
build.
10.1 Getting EnsDb databases
Some EnsDb databases are available as R packages from Bioconductor and can be
simply installed with the install function from the BiocManager package. The
name of such annotation packages starts with EnsDb followed by the abbreviation
of the organism and the Ensembl version on which the annotation
bases. EnsDb.Hsapiens.v86 provides thus an EnsDb database for homo sapiens with
annotations from Ensembl version 86.
Since Bioconductor version 3.5 EnsDb databases can also be retrieved directly
from AnnotationHub.
library(AnnotationHub)
## Load the annotation resource.
ah <- AnnotationHub()
## Query for all available EnsDb databases
query(ah, "EnsDb")## AnnotationHub with 1321 records
## # snapshotDate(): 2019-10-29
## # $dataprovider: Ensembl
## # $species: Homo sapiens, Ailuropoda melanoleuca, Anolis carolinensis, Astya...
## # $rdataclass: EnsDb
## # additional mcols(): taxonomyid, genome, description,
## # coordinate_1_based, maintainer, rdatadateadded, preparerclass, tags,
## # rdatapath, sourceurl, sourcetype
## # retrieve records with, e.g., 'object[["AH53185"]]'
##
## title
## AH53185 | Ensembl 87 EnsDb for Anolis Carolinensis
## AH53186 | Ensembl 87 EnsDb for Ailuropoda Melanoleuca
## AH53187 | Ensembl 87 EnsDb for Astyanax Mexicanus
## AH53188 | Ensembl 87 EnsDb for Anas Platyrhynchos
## AH53189 | Ensembl 87 EnsDb for Bos Taurus
## ... ...
## AH75113 | Ensembl 98 EnsDb for Vulpes vulpes
## AH75114 | Ensembl 98 EnsDb for Xiphophorus couchianus
## AH75115 | Ensembl 98 EnsDb for Xiphophorus maculatus
## AH75116 | Ensembl 98 EnsDb for Xenopus tropicalis
## AH75117 | Ensembl 98 EnsDb for Zonotrichia albicollisWe can simply fetch one of the databases.
ahDb <- query(ah, pattern = c("Xiphophorus Maculatus", "EnsDb", 87))
## What have we got
ahDb## AnnotationHub with 1 record
## # snapshotDate(): 2019-10-29
## # names(): AH53251
## # $dataprovider: Ensembl
## # $species: Xiphophorus maculatus
## # $rdataclass: EnsDb
## # $rdatadateadded: 2017-02-07
## # $title: Ensembl 87 EnsDb for Xiphophorus Maculatus
## # $description: Gene and protein annotations for Xiphophorus Maculatus based...
## # $taxonomyid: 8083
## # $genome: Xipmac4.4.2
## # $sourcetype: ensembl
## # $sourceurl: http://www.ensembl.org
## # $sourcesize: NA
## # $tags: c("EnsDb", "Ensembl", "Gene", "Transcript", "Protein",
## # "Annotation", "87", "AHEnsDbs")
## # retrieve record with 'object[["AH53251"]]'Fetch the EnsDb and use it.
ahEdb <- ahDb[[1]]
## retriebe all genes
gns <- genes(ahEdb)We could even make an annotation package from this EnsDb object using the
makeEnsembldbPackage and passing dbfile(dbconn(ahEdb)) as ensdb argument.
10.2 Building annotation packages
10.2.1 Directly from Ensembl databases
The fetchTablesFromEnsembl function uses the Ensembl Perl API
to retrieve the required annotations from an Ensembl database (e.g. from the
main site ensembldb.ensembl.org). Thus, to use this functionality to build
databases, the Ensembl Perl API needs to be installed (see 5 for details).
Below we create an EnsDb database by fetching the required data directly from
the Ensembl core databases. The makeEnsembldbPackage function is then used to
create an annotation package from this EnsDb containing all human genes for
Ensembl version 75.
library(ensembldb)
## get all human gene/transcript/exon annotations from Ensembl (75)
## the resulting tables will be stored by default to the current working
## directory
fetchTablesFromEnsembl(75, species = "human")
## These tables can then be processed to generate a SQLite database
## containing the annotations (again, the function assumes the required
## txt files to be present in the current working directory)
DBFile <- makeEnsemblSQLiteFromTables()
## and finally we can generate the package
makeEnsembldbPackage(ensdb = DBFile, version = "0.99.12",
maintainer = "Johannes Rainer <[email protected]>",
author = "J Rainer")The generated package can then be build using R CMD build EnsDb.Hsapiens.v75
and installed with R CMD INSTALL EnsDb.Hsapiens.v75*. Note that we could
directly generate an EnsDb instance by loading the database file, i.e. by
calling edb <- EnsDb(DBFile) and work with that annotation object.
To fetch and build annotation packages for plant genomes (e.g. arabidopsis
thaliana), the Ensembl genomes should be specified as a host, i.e. setting
host to “mysql-eg-publicsql.ebi.ac.uk”, port to 4157 and species to
e.g. “arabidopsis thaliana”.
10.2.2 From a GTF or GFF file
Alternatively, the ensDbFromAH, ensDbFromGff, ensDbFromGRanges and ensDbFromGtf
functions allow to build EnsDb SQLite files from a GRanges object or GFF/GTF
files from Ensembl (either provided as files or via AnnotationHub). These
functions do not depend on the Ensembl Perl API, but require a working internet
connection to fetch the chromosome lengths from Ensembl as these are not
provided within GTF or GFF files. Also note that protein annotations are usually
not available in GTF or GFF files, thus, such annotations will not be included
in the generated EnsDb database - protein annotations are only available in
EnsDb databases created with the Ensembl Perl API (such as the ones provided
through AnnotationHub or as Bioconductor packages).
In the next example we create an EnsDb database using the AnnotationHub package
and load also the corresponding genomic DNA sequence matching the Ensembl
version. We thus first query the AnnotationHub package for all resources
available for Mus musculus and the Ensembl release 77. Next we create the EnsDb
object from the appropriate AnnotationHub resource. We then use the
getGenomeTwoBitFile method on the EnsDb to directly look up and retrieve the
correct or best matching TwoBitFile with the genomic DNA sequence. At last we
retrieve the sequences of all exons using the getSeq method.
## Load the AnnotationHub data.
library(AnnotationHub)
ah <- AnnotationHub()
## Query all available files for Ensembl release 77 for
## Mus musculus.
query(ah, c("Mus musculus", "release-77"))
## Get the resource for the gtf file with the gene/transcript definitions.
Gtf <- ah["AH28822"]
## Create a EnsDb database file from this.
DbFile <- ensDbFromAH(Gtf)
## We can either generate a database package, or directly load the data
edb <- EnsDb(DbFile)
## Identify and get the TwoBit object with the genomic DNA sequence matching
## the EnsDb annotation.
Dna <- getGenomeTwoBitFile(edb)
## We next retrieve the sequence of all exons on chromosome Y.
exons <- exons(edb, filter = SeqNameFilter("Y"))
exonSeq <- getSeq(Dna, exons)In the example below we load a GRanges containing gene definitions for genes
encoded on chromosome Y and generate a EnsDb SQLite database from that
information.
## Generate a sqlite database from a GRanges object specifying
## genes encoded on chromosome Y
load(system.file("YGRanges.RData", package = "ensembldb"))
Y## GRanges object with 7155 ranges and 16 metadata columns:
## seqnames ranges strand | source type
## <Rle> <IRanges> <Rle> | <factor> <factor>
## [1] Y 2652790-2652894 + | snRNA gene
## [2] Y 2652790-2652894 + | snRNA transcript
## [3] Y 2652790-2652894 + | snRNA exon
## [4] Y 2654896-2655740 - | protein_coding gene
## [5] Y 2654896-2655740 - | protein_coding transcript
## ... ... ... ... . ... ...
## [7151] Y 28772667-28773306 - | processed_pseudogene transcript
## [7152] Y 28772667-28773306 - | processed_pseudogene exon
## [7153] Y 59001391-59001635 + | pseudogene gene
## [7154] Y 59001391-59001635 + | processed_pseudogene transcript
## [7155] Y 59001391-59001635 + | processed_pseudogene exon
## score phase gene_id gene_name gene_source
## <numeric> <integer> <character> <character> <character>
## [1] <NA> <NA> ENSG00000251841 RNU6-1334P ensembl
## [2] <NA> <NA> ENSG00000251841 RNU6-1334P ensembl
## [3] <NA> <NA> ENSG00000251841 RNU6-1334P ensembl
## [4] <NA> <NA> ENSG00000184895 SRY ensembl_havana
## [5] <NA> <NA> ENSG00000184895 SRY ensembl_havana
## ... ... ... ... ... ...
## [7151] <NA> <NA> ENSG00000231514 FAM58CP havana
## [7152] <NA> <NA> ENSG00000231514 FAM58CP havana
## [7153] <NA> <NA> ENSG00000235857 CTBP2P1 havana
## [7154] <NA> <NA> ENSG00000235857 CTBP2P1 havana
## [7155] <NA> <NA> ENSG00000235857 CTBP2P1 havana
## gene_biotype transcript_id transcript_name transcript_source
## <character> <character> <character> <character>
## [1] snRNA <NA> <NA> <NA>
## [2] snRNA ENST00000516032 RNU6-1334P-201 ensembl
## [3] snRNA ENST00000516032 RNU6-1334P-201 ensembl
## [4] protein_coding <NA> <NA> <NA>
## [5] protein_coding ENST00000383070 SRY-001 ensembl_havana
## ... ... ... ... ...
## [7151] pseudogene ENST00000435741 FAM58CP-001 havana
## [7152] pseudogene ENST00000435741 FAM58CP-001 havana
## [7153] pseudogene <NA> <NA> <NA>
## [7154] pseudogene ENST00000431853 CTBP2P1-001 havana
## [7155] pseudogene ENST00000431853 CTBP2P1-001 havana
## exon_number exon_id tag ccds_id protein_id
## <numeric> <character> <character> <character> <character>
## [1] <NA> <NA> <NA> <NA> <NA>
## [2] <NA> <NA> <NA> <NA> <NA>
## [3] 1 ENSE00002088309 <NA> <NA> <NA>
## [4] <NA> <NA> <NA> <NA> <NA>
## [5] <NA> <NA> CCDS CCDS14772 <NA>
## ... ... ... ... ... ...
## [7151] <NA> <NA> <NA> <NA> <NA>
## [7152] 1 ENSE00001616687 <NA> <NA> <NA>
## [7153] <NA> <NA> <NA> <NA> <NA>
## [7154] <NA> <NA> <NA> <NA> <NA>
## [7155] 1 ENSE00001794473 <NA> <NA> <NA>
## -------
## seqinfo: 1 sequence from GRCh37 genome## Create the EnsDb database file
DB <- ensDbFromGRanges(Y, path = tempdir(), version = 75,
organism = "Homo_sapiens")## Warning in ensDbFromGRanges(Y, path = tempdir(), version = 75, organism =
## "Homo_sapiens"): I'm missing column(s): 'entrezid'. The corresponding database
## column(s) will be empty!## Load the database
edb <- EnsDb(DB)
edb## EnsDb for Ensembl:
## |Backend: SQLite
## |Db type: EnsDb
## |Type of Gene ID: Ensembl Gene ID
## |Supporting package: ensembldb
## |Db created by: ensembldb package from Bioconductor
## |script_version: 0.0.1
## |Creation time: Wed Nov 20 21:08:48 2019
## |ensembl_version: 75
## |ensembl_host: unknown
## |Organism: Homo_sapiens
## |genome_build: GRCh37
## |DBSCHEMAVERSION: 1.0
## |source_file: GRanges object
## | No. of genes: 495.
## | No. of transcripts: 731.Alternatively we can build the annotation database using the ensDbFromGtf
ensDbFromGff functions, that extract most of the required data from a GTF
respectively GFF (version 3) file which can be downloaded from Ensembl
(e.g. from ftp://ftp.ensembl.org/pub/release-75/gtf/homo_sapiens for human gene
definitions from Ensembl version 75; for plant genomes etc, files can be
retrieved from ftp://ftp.ensemblgenomes.org). All information except the
chromosome lengths, the NCBI Entrezgene IDs and protein annotations can be
extracted from these GTF files. The function also tries to retrieve chromosome
length information automatically from Ensembl.
Below we create the annotation from a gtf file that we fetch directly from Ensembl.
library(ensembldb)
## the GTF file can be downloaded from
## ftp://ftp.ensembl.org/pub/release-75/gtf/homo_sapiens/
gtffile <- "Homo_sapiens.GRCh37.75.gtf.gz"
## generate the SQLite database file
DB <- ensDbFromGtf(gtf = gtffile)
## load the DB file directly
EDB <- EnsDb(DB)
## alternatively, build the annotation package
## and finally we can generate the package
makeEnsembldbPackage(ensdb = DB, version = "0.99.12",
maintainer = "Johannes Rainer <[email protected]>",
author = "J Rainer")11 Database layout
The database consists of the following tables and attributes (the layout is also
shown in Figure 165). Note that the protein-specific annotations
might not be available in all EnsDB databases (e.g. such ones created with
ensembldb version < 1.7 or created from GTF or GFF files).
- gene: all gene specific annotations.
gene_id: the Ensembl ID of the gene.gene_name: the name (symbol) of the gene.gene_biotype: the biotype of the gene.gene_seq_start: the start coordinate of the gene on the sequence (usually a chromosome).gene_seq_end: the end coordinate of the gene on the sequence.seq_name: the name of the sequence (usually the chromosome name).seq_strand: the strand on which the gene is encoded.seq_coord_system: the coordinate system of the sequence.description: the description of the gene.
- entrezgene: mapping of Ensembl genes to NCBI Entrezgene identifiers. Note that
this mapping can be a one-to-many mapping.
gene_id: the Ensembl gene ID.entrezid: the NCBI Entrezgene ID.
tx: all transcript related annotations. Note that while no
tx_namecolumn is available in this database column, all methods to retrieve data from the database support also this column. The returned values are however the ID of the transcripts.tx_id: the Ensembl transcript ID.tx_biotype: the biotype of the transcript.tx_seq_start: the start coordinate of the transcript.tx_seq_end: the end coordinate of the transcript.tx_cds_seq_start: the start coordinate of the coding region of the transcript (NULL for non-coding transcripts).tx_cds_seq_end: the end coordinate of the coding region of the transcript.gene_id: the gene to which the transcript belongs.
EnsDbdatabases for more recent Ensembl releases have also a columntx_support_levelproviding the evidence level for a transcript (1 high evidence, 5 low evidence, NA no evidence calculated).- exon: all exon related annotation.
exon_id: the Ensembl exon ID.exon_seq_start: the start coordinate of the exon.exon_seq_end: the end coordinate of the exon.
- tx2exon: provides the n:m mapping between transcripts and exons.
tx_id: the Ensembl transcript ID.exon_id: the Ensembl exon ID.exon_idx: the index of the exon in the corresponding transcript, always from 5’ to 3’ of the transcript.
- chromosome: provides some information about the chromosomes.
seq_name: the name of the sequence/chromosome.seq_length: the length of the sequence.is_circular: whether the sequence in circular.
- protein: provides protein annotation for a (coding) transcript.
protein_id: the Ensembl protein ID.tx_id: the transcript ID which CDS encodes the protein.protein_sequence: the peptide sequence of the protein (translated from the transcript’s coding sequence after applying eventual RNA editing).
- uniprot: provides the mapping from Ensembl protein ID(s) to Uniprot ID(s). Not
all Ensembl proteins are annotated to Uniprot IDs, also, each Ensembl protein
might be mapped to multiple Uniprot IDs.
protein_id: the Ensembl protein ID.uniprot_id: the Uniprot ID.uniprot_db: the Uniprot database in which the ID is defined.uniprot_mapping_type: the type of the mapping method that was used to assign the Uniprot ID to an Ensembl protein ID.
- protein_domain: provides protein domain annotations and mapping to proteins.
protein_id: the Ensembl protein ID on which the protein domain is present.protein_domain_id: the ID of the protein domain (from the protein domain source).protein_domain_source: the source/analysis method in/by which the protein domain was defined (such as pfam etc).interpro_accession: the Interpro accession ID of the protein domain.prot_dom_start: the start position of the protein domain within the protein’s sequence.prot_dom_end: the end position of the protein domain within the protein’s sequence.
- metadata: some additional, internal, informations (Genome build, Ensembl
version etc).
namevalue
- virtual columns:
symbol: the database does not have such a database column, but it is still possible to use it in thecolumnsparameter. This column is symlinked to thegene_namecolumn.tx_name: similar to thesymbolcolumn, this column is symlinked to thetx_idcolumn.
The database layout: as already described above, protein related annotations
(green) might not be available in each EnsDb database.
img
12 Session information
sessionInfo()## R version 3.6.1 (2019-07-05)
## Platform: x86_64-pc-linux-gnu (64-bit)
## Running under: Ubuntu 18.04.3 LTS
##
## Matrix products: default
## BLAS: /home/biocbuild/bbs-3.10-bioc/R/lib/libRblas.so
## LAPACK: /home/biocbuild/bbs-3.10-bioc/R/lib/libRlapack.so
##
## locale:
## [1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C
## [3] LC_TIME=en_US.UTF-8 LC_COLLATE=C
## [5] LC_MONETARY=en_US.UTF-8 LC_MESSAGES=en_US.UTF-8
## [7] LC_PAPER=en_US.UTF-8 LC_NAME=C
## [9] LC_ADDRESS=C LC_TELEPHONE=C
## [11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C
##
## attached base packages:
## [1] grid stats4 parallel stats graphics grDevices utils
## [8] datasets methods base
##
## other attached packages:
## [1] AnnotationHub_2.18.0
## [2] BiocFileCache_1.10.2
## [3] dbplyr_1.4.2
## [4] magrittr_1.5
## [5] BSgenome.Hsapiens.NCBI.GRCh38_1.3.1000
## [6] BSgenome_1.54.0
## [7] rtracklayer_1.46.0
## [8] Biostrings_2.54.0
## [9] XVector_0.26.0
## [10] Gviz_1.30.0
## [11] EnsDb.Hsapiens.v86_2.99.0
## [12] ensembldb_2.10.2
## [13] AnnotationFilter_1.10.0
## [14] GenomicFeatures_1.38.0
## [15] AnnotationDbi_1.48.0
## [16] Biobase_2.46.0
## [17] GenomicRanges_1.38.0
## [18] GenomeInfoDb_1.22.0
## [19] IRanges_2.20.1
## [20] S4Vectors_0.24.0
## [21] BiocGenerics_0.32.0
## [22] BiocStyle_2.14.0
##
## loaded via a namespace (and not attached):
## [1] ProtGenerics_1.18.0 bitops_1.0-6
## [3] matrixStats_0.55.0 bit64_0.9-7
## [5] RColorBrewer_1.1-2 progress_1.2.2
## [7] httr_1.4.1 tools_3.6.1
## [9] backports_1.1.5 R6_2.4.1
## [11] rpart_4.1-15 Hmisc_4.3-0
## [13] DBI_1.0.0 lazyeval_0.2.2
## [15] colorspace_1.4-1 nnet_7.3-12
## [17] tidyselect_0.2.5 gridExtra_2.3
## [19] prettyunits_1.0.2 bit_1.1-14
## [21] curl_4.2 compiler_3.6.1
## [23] htmlTable_1.13.2 DelayedArray_0.12.0
## [25] bookdown_0.15 scales_1.1.0
## [27] checkmate_1.9.4 askpass_1.1
## [29] rappdirs_0.3.1 stringr_1.4.0
## [31] digest_0.6.22 Rsamtools_2.2.1
## [33] foreign_0.8-72 rmarkdown_1.17
## [35] dichromat_2.0-0 base64enc_0.1-3
## [37] pkgconfig_2.0.3 htmltools_0.4.0
## [39] fastmap_1.0.1 highr_0.8
## [41] htmlwidgets_1.5.1 rlang_0.4.1
## [43] rstudioapi_0.10 RSQLite_2.1.2
## [45] shiny_1.4.0 BiocParallel_1.20.0
## [47] acepack_1.4.1 dplyr_0.8.3
## [49] VariantAnnotation_1.32.0 RCurl_1.95-4.12
## [51] GenomeInfoDbData_1.2.2 Formula_1.2-3
## [53] Matrix_1.2-17 Rcpp_1.0.3
## [55] munsell_0.5.0 lifecycle_0.1.0
## [57] stringi_1.4.3 yaml_2.2.0
## [59] SummarizedExperiment_1.16.0 zlibbioc_1.32.0
## [61] blob_1.2.0 promises_1.1.0
## [63] crayon_1.3.4 lattice_0.20-38
## [65] splines_3.6.1 hms_0.5.2
## [67] zeallot_0.1.0 knitr_1.26
## [69] pillar_1.4.2 biomaRt_2.42.0
## [71] BiocVersion_3.10.1 XML_3.98-1.20
## [73] glue_1.3.1 evaluate_0.14
## [75] biovizBase_1.34.0 latticeExtra_0.6-28
## [77] data.table_1.12.6 BiocManager_1.30.10
## [79] httpuv_1.5.2 vctrs_0.2.0
## [81] gtable_0.3.0 openssl_1.4.1
## [83] purrr_0.3.3 assertthat_0.2.1
## [85] ggplot2_3.2.1 xfun_0.11
## [87] mime_0.7 xtable_1.8-4
## [89] later_1.0.0 survival_3.1-7
## [91] tibble_2.1.3 GenomicAlignments_1.22.1
## [93] memoise_1.1.0 cluster_2.1.0
## [95] interactiveDisplayBase_1.24.0